Effect of temperature variation on the cytotoxicity of cast dental alloys and commercially pure titanium.

Journal of Applied Oral Science : Revista FOB
Adriana Cláudia Lapria FariaRicardo Faria Ribeiro

Abstract

Cell culture system has been used to evaluate alloy cytotoxicity under different environments, testing the extracts, but the effect of temperature variation on the cytotoxicity of dental alloys has not been analyzed. The aim of the present study was to investigate if temperature variation could affect dental alloy cytotoxicity, testing alloy extracts in an epithelial cell culture system. Discs of Ni-Cr, Co-Cr-Mo, Ni-Cr-Ti, Ti-6Al-4V and commercially pure titanium (cp Ti) were cast by arc melting, under argon atmosphere, injected by vacuum-pressure. Discs were immersed in artificial saliva and subjected to different temperatures: 37 degrees C and thermocycling (37 degrees C/5 degrees C/37 degrees C/55 degrees C/37 degrees C). After thermocycling, extracts were put in a subconfluent culture during 6 h, and the number of cells and their viability were used to evaluate cytotoxicity in these temperatures. For each alloy, data from temperature conditions were compared by Student's t-test (alpha=0.05). The cytotoxicity tests with alloy/metal extracts showed that Ni-Cr, Co-Cr-Mo, Ti-6Al-4V and cp Ti extracts (p>0.05) did not affect cell number or cell viability, while Ni-Cr-Ti (p<0.05) extract decreased cell number and viability when t...Continue Reading

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Citations

Apr 9, 2013·Journal of Materials Science. Materials in Medicine·Lufeng YuZana Mariano
Aug 5, 2014·The Journal of Prosthetic Dentistry·Adriana C L FariaRicardo F Ribeiro
Nov 5, 2011·Journal of Vascular Surgery·Nitin GargManju Kalra
Mar 10, 2015·The Journal of Prosthetic Dentistry·Paulo Cézar Simamoto JúniorLuís Henrique Araújo Raposo
Mar 2, 2011·Brazilian Oral Research·Juliana Maria Costa Nuñez-PantojaMarcelo Ferraz Mesquita
Jan 29, 2011·Brazilian Dental Journal·Gustavo Augusto Seabra BarbosaRicardo Faria Ribeiro
Dec 28, 2017·The Journal of Advanced Prosthodontics·Jung-Hwan LeeHae-Hyoung Lee
Apr 25, 2017·Gerodontology·Denise Tornavoi de CastroAndréa Cândido Dos Reis
Nov 30, 2019·Biological Trace Element Research·Rupali AgnihotriSacharia Albin

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