Effect of tertiary butylhydroperoxide-induced oxidative stress on glutathione content and thiobarbituric acid reactive substances production in cultures and co-cultures of adult rat hepatocytes

Toxicology in Vitro : an International Journal Published in Association with BIBRA
K MertensV Rogiers

Abstract

The production of thiobarbituric acid reactive substances (TBARS) and the content of reduced glutathione (GSH) were determined in primary monolayer cultures of adult rat hepatocytes and in their co-cultures with rat epithelial cells, both exposed for 90 min to 2 mm tertiary butylhydroperoxide. Under these stress-induced conditions, TBARS production tended to be less increased in co-cultured cells than in conventionally cultured hepatocytes. A total depletion of GSH content was measured in conventional cultures from the third culture day, whereas in co-cultures a decrease in GSH content was observed until the third day of culture, followed by a low but constant GSH level that was maintained until the end of the culture period. Oxidative stress appears to be less pronounced in co-cultures than in conventional cultures; the former cope better with externally induced oxidative stress than do the latter.

References

Jan 1, 1978·Biochemical Pharmacology·Y Masuda, T Murano
Apr 19, 1991·Journal of Chromatography·K MertensA Vercruysse
Sep 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·V RogiersA Vercruysse
Nov 1, 1971·Experimental Cell Research·G M WilliamsJ H Weisburger
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·K MertensA Vercruysse

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Citations

Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T VanhaeckeV Rogiers
Mar 11, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nahla S El-Shenawy
Apr 30, 2005·Journal of Medical Primatology·Rachel E Cianciolo, Gene B Hubbard
Jul 1, 1999·Alternatives to Laboratory Animals : ATLA·S CoeckeC Westmoreland

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