PMID: 2510827Nov 20, 1989Paper

Effect of testicular hyaluronidase on hyaluronate synthesis by human skin fibroblasts in culture

Biochimica Et Biophysica Acta
C LarnierM Moczar

Abstract

The effect of hyaluronidase treatment on the incorporation of [3H]glucosamine into hyaluronate in human skin fibroblast cultures was investigated. Fourth passage cells in confluent cultures were treated with hyaluronidase from bovine tests, Streptomyces and leech in Dulbecco's minimum essential medium in the presence of 3% fetal calf serum. The medium was removed from the control (non-treated) and the treated cultures and the washed cell layers were incubated with [3H]glucosamine and [35S]sulfate. [3H]Hyaluronate was separated by DEAE Trisacyl chromatography and identified by specific enzymic assays. Hyaluronidase treatment induced an increase in the amount of labelled hyaluronate secreted into the medium and into the pericellular compartment. This amount reached a plateau with increasing enzyme concentration and with the time of treatment. Oligosaccharides derived from hyaluronate did not produce this effect. The maximal increase was about 3-fold, and was not inhibited by exogenous hyaluronate (25-100 micrograms/ml) or by oligosaccharides from hyaluronate. Cycloheximide (0.03 mM) inhibited hyaluronate synthesis by 18% or less in the control cells and by 50% in the hyaluronidase-pretreated fibroblasts. No significant difference...Continue Reading

References

Apr 1, 1979·The Journal of Cell Biology·K HedmanM Höök
Sep 1, 1978·The Journal of Surgical Research·C N Bertolami, R B Donoff
Sep 23, 1977·Biochemical and Biophysical Research Communications·W TruppeH Kresse
Oct 15, 1986·The Biochemical Journal·M BrechtP Prehm
Oct 1, 1985·Scandinavian Journal of Clinical and Laboratory Investigation·A Engström-LaurentT C Laurent
Oct 18, 1969·Lancet·R Matalon, A Dorfman
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J R HassellG R Martin
Jun 1, 1984·The Biochemical Journal·P Prehm
Jan 15, 1982·The Biochemical Journal·D Mitchell, T Hardingham
Jan 15, 1982·Analytical Biochemistry·M HöökU Lindahl
Jan 15, 1981·The Biochemical Journal·P J GillJ E Silbert
Nov 17, 2011·The Journal of Arthroplasty·Lucian B SolomonDonald W Howie

❮ Previous
Next ❯

Citations

Jan 1, 1992·Journal of Cancer Research and Clinical Oncology·K BeckenlehnerW Schiess
Sep 15, 1990·International Journal of Cancer. Journal International Du Cancer·B DelpechB Asselain
Jan 1, 1994·Progress in Histochemistry and Cytochemistry·R TammiM Tammi
Mar 18, 2006·The Journal of Investigative Dermatology·Sri Rajalakshmi RudrabhatlaMark E Mummert
Aug 1, 1990·FEBS Letters·J Labat-RobertL Robert
Nov 25, 2020·European Journal of Medical Research·Bettina Alexandra BuhrenPeter Arne Gerber
Oct 27, 2004·European Journal of Cell Biology·Robert Stern

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.