Abstract
Botulinum neurotoxin serotypes A, B and E were modified at pH 7.9 with tetranitromethane, a reagent highly specific for tyrosine residues. The type B and E neurotoxins were completely detoxified without significant damage to their serological activities. Under similar modification conditions, the type A neurotoxin was incompletely detoxified with some alteration in its serological reactivity. Modification of only tyrosine residues to nitrotyrosine was evident from amino acid analysis of the acid hydrolysates of the modified proteins. The completely detoxified type B and E neurotoxins, used as toxoid, elicited antibodies in rabbits. The antisera precipitated and neutralized the homologous neurotoxin. The two toxoids, type B and E, were prepared with greater than 99% pure neurotoxins as tested by sodium dodecyl sulfate-polyacrylamide gel electrophoresis whereas the traditional toxoids produced with formaldehyde are very crude preparations of the neurotoxin (approximately 90% impure). Chemical modification using tetranitromethane is more specific than products that form during approximately 7 days of reaction between a protein and formaldehyde. The toxoids produced with tetranitromethane may be considered second-generation toxoids...Continue Reading
References
Sep 1, 1977·Canadian Journal of Microbiology·B R DasGupta, H Sugiyama
May 1, 1975·Infection and Immunity·S Kozaki, G Sakaguchi
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·B R Singh, B R DasGupta
Apr 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·H SugiyamaI Oishi
Dec 27, 1968·Science·P CuatrecasasC B Anfinsen
Aug 21, 1970·Biochimica Et Biophysica Acta·J KnoxL Spero
Jul 11, 1972·Biochemical and Biophysical Research Communications·B R DasGupta, H Sugiyama
Jun 1, 1971·Japanese Journal of Medical Science & Biology·K AkamaS Sadahiro
Jun 1, 1971·Japanese Journal of Medical Science & Biology·K AkamaR Murata
Nov 1, 1966·Journal of Bacteriology·D A Boroff, U Fleck
Apr 25, 1966·Biochimica Et Biophysica Acta·D A Boroff, B R Dasgupta
Feb 1, 1982·Biochimie·L M Houdebine
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·B R DasGupta, S Rasmussen
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·B R DasGupta, V Sathyamoorthy
Jul 1, 1984·Archives of Biochemistry and Biophysics·B R Dasgupta, S Rasmussen
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·B R DasGupta, M A Woody
Jan 1, 1982·Pharmacology & Therapeutics·G Sakaguchi
Aug 31, 1981·Biochemical and Biophysical Research Communications·B R DasGupta, S Rasmussen
Feb 1, 1982·Biochimie·F Le GaillardM Dautrevaux
Mar 1, 1982·The Biochemical Journal·J Williams
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee
Citations
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·M A WoodyB R DasGupta
Aug 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·S PrabakaranB R DasGupta
Oct 23, 1998·Nature Structural Biology·D B LacyR C Stevens
Mar 21, 2007·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yoshiaki KasaiTateo Fujii
Jun 24, 2008·Journal of Immunological Methods·Russell G A JonesDorothea Sesardic
Nov 1, 1989·Biophysical Chemistry·B R Singh, B R DasGupta
Oct 1, 1990·Biophysical Chemistry·B R Singh, B R DasGupta
Mar 8, 1991·Biochimica Et Biophysica Acta·B R SinghB R DasGupta