Effect of the air-water interface on the stability of beta-lactoglobulin

The Journal of Physical Chemistry. B
Adam W PerrimanJohn W White


We report the X-ray and neutron reflectometry measurements of the structural changes caused by chemical denaturation of a surface excess of the bovine milk protein, beta-lactoglobulin. The thickness of the diffuse protein surface layer was used as an order parameter as there was no corresponding increase in the surface excess as a function of guanidinium chloride (G.HCl) concentration. A thermodynamic analysis performed gave the interfacial free energy of unfolding in the absence of a denaturant (DeltaG(0)). This energy, lower than the free energy of unfolding bulk solution, shows that the air-water interface has a destabilizing effect on protein structure up to 50 kJ mol(-1).


Feb 18, 2010·International Journal of Nanomedicine·Mariagrazia Di MarcoClaudia Sadun
Jul 20, 2011·Journal of Oleo Science·Nopparat CheetangdeeKazuhiro Fukada
Apr 1, 2014·The Journal of Physical Chemistry. B·Kathrin EngelhardtBjörn Braunschweig
May 8, 2014·Advances in Colloid and Interface Science·Aleksandra Kezwon, Kamil Wojciechowski
Nov 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Richard A CampbellJohn W White
Jun 3, 2015·Colloids and Surfaces. B, Biointerfaces·Aleksandra KezwońKamil Wojciechowski
Mar 3, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Anton P J Middelberg, Mirjana Dimitrijev-Dwyer
Apr 11, 2013·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Anja ButtstedtElisabeth Schwarz
Apr 4, 2015·Colloids and Surfaces. B, Biointerfaces·M M TihonovB A Noskov
Apr 1, 2015·The Journal of Physical Chemistry. B·Frank R BeierleinWolfgang Peukert
Dec 23, 2009·Biochimica Et Biophysica Acta·Joseph A ZasadzinskiPrajna Dhar
Aug 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Kathrin EngelhardtBjörn Braunschweig
Mar 30, 2012·The Journal of Physical Chemistry. B·Marek PiotrowskiKamil Wojciechowski
Dec 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jiali ZhaiTim J Wooster
Jul 26, 2011·The Journal of Physical Chemistry. B·A A MikhailovskayaR Miller
Jun 30, 2019·Biotechnology Progress·Mafalda Dias GomesJohn M Woodley
Nov 21, 2012·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Yohko F Yano
May 5, 2017·Journal of the Royal Society, Interface·Anne-Christin SchöneAndreas Lendlein
Jan 5, 2021·Soft Matter·Jotam BergfreundPeter Fischer

Related Concepts

Protein Denaturation

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.