Effect of the development of a cell barrier on nanoparticle uptake in endothelial cells

Nanoscale
Valentina FranciaAnna Salvati

Abstract

In order to improve the current success of nanomedicine, a better understanding of how nano-sized materials interact with and are processed by cells is required. Typical in vitro nanoparticle-cell interaction studies often make use of cells cultured at different cell densities. However, in vivo, for their successful delivery to the target tissue, nanomedicines need to overcome several barriers, such as endothelial and epithelial cell barriers. Unlike sub-confluent or confluent cell cultures, cell barriers are tight cell monolayers, expressing a series of specialized tight junction proteins between adjacent cells to limit paracellular transport and ensure close cell-to-cell interactions. A clear understanding on how the development of cells into a cell barrier may affect the uptake of nano-sized drug carriers is still missing. To this aim, here, human primary umbilical vein endothelial cells (HUVEC) are used as a model cell line to form endothelial cell barriers. Then, nanoparticle uptake is assessed in the developed endothelial barriers and compared to the uptake in sub-confluent or confluent HUVEC cultures. The results clearly show that the organization of cells into a cell barrier leads to a differential gene expression of en...Continue Reading

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Citations

Jul 10, 2020·Small·Alessandro ParodiEnnio Tasciotti
Oct 1, 2020·Journal of Biomedical Materials Research. Part a·Qinghao ZhangIan Nettleship
Mar 3, 2020·Beilstein Journal of Nanotechnology·Valentina FranciaAnna Salvati
Nov 24, 2020·Virulence·Elisa J M RaineriJan Maarten van Dijl
Jun 26, 2021·Advanced Healthcare Materials·Christina Cortez-JugoFrank Caruso
Aug 12, 2021·Journal of Extracellular Vesicles·Ricardo C de AbreuLino Ferreira

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Methods Mentioned

BETA
Light
confocal microscopy
fluorescence microscopy
light microscopy
flow cytometry
transfect
GTPase
flow
PCR
dynamic light scattering

Software Mentioned

IMARIS
SDS
Flowjo
Flowjo®
ImageJ

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