PMID: 8589736Sep 1, 1995Paper

Effect of the dpy-20 and rol-6 cotransformation markers on alpha-tubulin gene expression in C. elegans transformants

Transgenic Research
T Fukushige, S S Siddiqui

Abstract

An alpha-1 tubulin::lacZ fusion gene was introduced into the germline of Caenorhabditis elegans, using either rol-6 or dpy-20 genomic DNA as a cotransformation marker. Distinct patterns in cellular specificity of the alpha-1 tubulin::lacZ fusion gene expression were observed, depending on the cotransformation marker used. For the rol-6 marker, the tubulin fusion gene was expressed in several neurons in the head and tail ganglia and a set of 38-39 ventral cord motor neurons along the body length of the animal during larval and adult development. In contrast, for the dpy-20 marker system, not only were fewer neurons stained in the head and tail region, but also the staining of ventral cord motor neurons was extremely reduced both in number and intensity. The dpy-20 marked-mediated suppression of the alpha-1 tubulin gene expression was observed both in the cis and trans configurations. Similar down-regulation in the ventral cord motor neurons was observed when the alpha-2 tubulin::lacZ fusion gene construct was tested in these experiments using the dpy-20 marker. In controls, where the tubulin fusion gene was directly injected to obtain transformants without any marker DNA, the cellular staining pattern was close to the fusion gen...Continue Reading

References

May 1, 1992·Nature Genetics·R WaterstonN Halloran
Apr 1, 1992·Genes & Development·M WesterfieldA W Püschel
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P CulpN Hopkins
Nov 18, 1988·Cell·N von MendeD L Riddle
Jan 1, 1986·Annual Review of Genetics·R D Palmiter, R L Brinster
Dec 1, 1985·Molecular and Cellular Biology·D T StinchcombD Hirsh
May 1, 1974·Genetics·S Brenner
Dec 20, 1993·Journal of Molecular Biology·T FukushigeS S Siddiqui
Jul 1, 1993·Transgenic Research·Y FurutaS Ishii
Jan 1, 1993·Transgenic Research·J M KrakowskyJ B Lingrel
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A CoulsonJ Karn
Feb 3, 1984·Science·R B HorschN Hoffmann
Nov 12, 1986·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G WhiteS Brenner

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Citations

Aug 9, 2002·Journal of Cellular and Molecular Medicine·Camelia Baleanu-Gogonea, S. S. Siddiqui

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