Effect of the fungal mycotoxin patulin on the chromatin structure of fission yeast Schizosaccharomyces pombe

Journal of Basic Microbiology
Eszter HorváthGáspár Bánfalvi

Abstract

The fungal mycotoxin patulin is produced by several molds, especially by Aspergillus and Penicillium. The aim of this study was to clarify whether patulin causes alterations in plasma membrane permeability of Schizosaccharomyces pombe lead to cellular shrinkage charateristic to apoptosis or increases cell size indicating necrosis in cells. Transmission and scanning electronmicroscopy revealed that lower concentrations of patulin induced cellular shrinkage and blebbing, higher concentration caused expansion without cellular disruption. Large-scale morphological changes of individual cells were followed by time lapse video microscopy. Patulin caused the elongation and stickiness of cells or rounded up their shapes. To visualize chromatin structures of S. pombe nuclei upon patulin treatment, protoplasts were isolated from S. pombe and subjected to fluorescent microscopy. Chromatin changes in the presence of 50 μM patulin concentration were characterized by elongated nuclei containing sticky fibrillary chromatin and enlarged round shaped nuclei trapped at the fibrillary stage of chromatin condensation. Short (60 min) incubation of S. pombe cells in the presence of high (500 μM) patulin concentration generated patches of condensed c...Continue Reading

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Citations

Apr 10, 2013·FEMS Yeast Research·Eduardo Campos-GóngoraGerman Larriba
Jan 12, 2016·Toxicon : Official Journal of the International Society on Toxinology·Gábor PappMiklós Pesti
Feb 17, 2015·Journal of Basic Microbiology·Walter P PflieglerIstván Pócsi
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Jul 17, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gábor PappMiklós Pesti
Oct 23, 2016·Apoptosis : an International Journal on Programmed Cell Death·Gabor NagyGaspar Banfalvi
Mar 3, 2020·Frontiers in Microbiology·Walter P PflieglerTünde Pusztahelyi

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis