Effect of the headgroup variation on the gene transfer properties of cholesterol based cationic lipids possessing ether linkage

Biochimica Et Biophysica Acta
Avinash BajajSantanu Bhattacharya

Abstract

Eight cholesterol based cationic lipids differing in the headgroup have been synthesized based on the ether linkage between the cationic headgroup and the cholesterol backbone. All the lipids formed stable suspensions in water. Transfection efficacies were examined in the absence and presence of serum using their optimized liposomal (lipid:DOPE) formulations. Our results showed that the transfection activities depend on the nature of the headgroup. Lipid bearing 4-N,N'-dimethylaminopyridine (DMAP) as headgroup showed the maximum transfection efficacy in the presence of serum. Importantly, the optimized formulation for this cationic lipid does not require DOPE, which is being used by most commercially available formulations. Cytotoxicity studies showed that the introduction of the positive charge decreases the cell viability of the cationic lipid formulations. Gel electrophoresis and Ethidium bromide exclusion assay revealed the different DNA binding abilities of formulations depending upon the headgroup of the cholesteryl lipid.

References

Nov 9, 1992·Biochimica Et Biophysica Acta·H FarhoodL Huang
Jun 11, 1992·Nature·A D Miller
Aug 30, 1991·Biochemical and Biophysical Research Communications·X Gao, L Huang
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P L FelgnerM Danielsen
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y YangJ M Wilson
Nov 17, 1995·Chemistry and Physics of Lipids·S BhattacharyaU S Hiremath
Aug 14, 1996·Biochimica Et Biophysica Acta·S Bhattacharya, S Haldar
Apr 16, 1998·Nature·C L AndersonB Okamura
May 17, 2001·Bioconjugate Chemistry· Han SoS W Kim
Aug 3, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·R I MahatoS W Kim
Apr 8, 2003·Current Medicinal Chemistry·Aaron HirkoJeffrey A Hughes
Apr 8, 2003·Current Medicinal Chemistry·Nejat DüzgüneşMaria C Pedroso de Lima
Apr 8, 2003·Current Medicinal Chemistry·M C Pedroso de LimaS Simões
Apr 8, 2003·Current Medicinal Chemistry·Valluripalli Vinod KumarArabinda Chaudhuri
Apr 8, 2003·Current Medicinal Chemistry·Dexi LiuXiang Gao
Apr 17, 2003·Angewandte Chemie·Anthony J KirbyMarcel C P van Eijk
Jul 17, 2003·Bioconjugate Chemistry·Darin Y FurgesonSung Wan Kim
May 20, 2004·Bioconjugate Chemistry·Santanu Bhattacharya, Padinjarae Vangasseri Dileep
Jul 9, 2004·Journal of Medicinal Chemistry·Marc Antoniu IliesAlexandru T Balaban
Sep 30, 2004·Journal of the American Chemical Society·Carla A H PrataMark W Grinstaff
Feb 4, 2005·Journal of Medicinal Chemistry·Joyeeta Sen, Arabinda Chaudhuri
Aug 5, 2005·Journal of Medicinal Chemistry·Cecilia BombelliMaria Grazia Sacco
Aug 11, 2005·Journal of the American Chemical Society·Chandrashekhar ChittimallaJean-Paul Behr
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Cecilia BombelliMaria Grazia Sacco
Mar 15, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Christopher M JewellDavid M Lynn
Mar 16, 2006·Bioconjugate Chemistry·Pauline ChabaudMark W Grinstaff
Feb 21, 2007·The Journal of Physical Chemistry. B·Santanu Bhattacharya, Avinash Bajaj

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Citations

Dec 14, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oleg O MarkovMarina A Zenkova
May 16, 2017·Gene Therapy·C ZylberbergS Matosevic
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Sep 24, 2013·Organic & Biomolecular Chemistry·Ekaterina A IvanovaMarina A Zenkova
Jun 16, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Matthias DittrichGerald Brezesinski
Jul 13, 2012·Chemical Biology & Drug Design·Shantal DorasamyMario Ariatti
Jan 12, 2019·Pharmaceutical Development and Technology·Upendra BulbakeWahid Khan
Apr 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Giuseppe VitielloLuigi Paduano
Oct 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Santanu Bhattacharya, Joydeep Biswas
Jul 12, 2008·Bioconjugate Chemistry·Avinash BajajSantanu Bhattacharya
Mar 7, 2013·Bioconjugate Chemistry·Defu ZhiDefeng Zhao
Feb 7, 2015·Journal of Medicinal Chemistry·Bogdan Draghici, Marc A Ilies
Jul 17, 2014·The Journal of Physical Chemistry. B·Priyanshu BhargavaAvinash Bajaj
Nov 4, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Bappa Maiti, Santanu Bhattacharya
Oct 15, 2010·Carbohydrate Research·Mikhail A MaslovGalina A Serebrennikova

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