PMID: 11322202Apr 27, 2001Paper

Effect of the hepatitis B virus HBx protein on integrin-mediated adhesion to and migration on extracellular matrix

Journal of Hepatology
Enrique Lara-PezziManuel López-Cabrera

Abstract

The hepatitis B virus HBx protein is associated with the development of hepatocellular carcinoma (HCC). However, its possible contribution to tumor spreading has not been explored. The migration of tumor cells through the extracellular matrix (ECM) represents a crucial step in tumor metastasis. Our aim was to study the effect of HBx on the integrin-mediated cell-ECM interaction, and its possible consequences for cell migration. Cell-ECM interaction was evaluated by static adhesion experiments, using blocking and stimulating anti-beta1 integrin mAbs. ECM receptor expression was analyzed by flow cytometry. The cellular distribution of the activated beta1 integrin subunit was determined by immunofluorescence analysis, and cell motility was determined by wound-healing assays. HBx-bearing cells showed decreased adhesion to fibronectin, which correlated with a decreased expression of the alpha5 integrin subunit. The activated beta1 subunit was redistributed to the tips of pseudopodial protrusions of HBx-bearing cells, whereas it was evenly localized in the control cells. HBx-induced cell migration was abrogated by irreversible stimulation of beta1 integrins. These results suggest that HBx might play a role in tumor spreading by modul...Continue Reading

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Citations

Aug 24, 2012·Journal of Cardiovascular Translational Research·Kalyani D PanseEnrique Lara-Pezzi
Aug 8, 2006·Postgraduate Medical Journal·N H ParkY-H Chung
Dec 1, 2007·Gut and Liver·Neung Hwa ParkYoung-Hwa Chung
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May 2, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Samuel Martín-VílchezEnrique Lara-Pezzi
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