PMID: 6160663Sep 1, 1980Paper

Effect of the hydrolyzing solution temperature, acid concentration and the duration of the hydrolysis on the intensity of the Feulgen reaction

Tsitologiia
Iu A MagakianA S Avetisian

Abstract

The quantity of DNA-fuchsin in the cock monocytes and erythrocytes has been measured by scanning cytophotometric methods and computer analysis. Hydrolysis was carried out using 1 N HCl (60 degrees C, 4-30 min) and 5 N HCl (37 degrees C, 4-36 min, and 22 degrees C, 10-150 min). The elevation of the temperature from 22 degrees to 37 degrees resulted in a 5-fold reducing of the time required for achieving the maximum of the hydrolysis curve, although the DNA content at the maximum point decreased by 7-9%. At 60 degrees and 1 N HCl, the loss of DNA reaches up to 30%. The prolongation of the hydrolysis time caused even more losses of DNA: at 60 degrees they are equal to 70% (22 minutes following the maximum point), at 37 degrees it is equal to 55% (26 minutes) and at 22 degrees only 9.5% (100 minutes) (the quantity of DNA at the maximum point is taken for 100%). During all the experimental conditions and in both the cell types, the hydrolysis curves are monomeacked, and at 22 degrees starting from 30 minutes a plateau is observed with a slight increase towards the 50th minute. The quantity of DNA-fuchsin in the loose nuclei of monocytes is generally higher than in compact nuclei of erythrocytes. The analysis of scanning- and histogr...Continue Reading

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