Effect of the Hydrophilic-Hydrophobic Balance of Antigen-Loaded Peptide Nanofibers on Their Cellular Uptake, Cellular Toxicity, and Immune Stimulatory Properties

International Journal of Molecular Sciences
Tomonori WakuNaoki Tanaka

Abstract

Recently, nanofibers (NFs) formed from antigenic peptides conjugated to β-sheet-forming peptides have attracted much attention as a new generation of vaccines. However, studies describing how the hydrophilic-hydrophobic balance of NF components affects cellular interactions of NFs are limited. In this report, three different NFs were prepared by self-assembly of β-sheet-forming peptides conjugated with model antigenic peptides (SIINFEKL) from ovalbumin and hydrophilic oligo-ethylene glycol (EG) of differing chain lengths (6-, 12- and 24-mer) to investigate the effect of EG length of antigen-loaded NFs on their cellular uptake, cytotoxicity, and dendritic cell (DC)-stimulation ability. We used an immortal DC line, termed JAWS II, derived from bone marrow-derived DCs of a C57BL/6 p53-knockout mouse. The uptake of NFs, consisting of the EG 12-mer by DCs, was the most effective and activated DC without exhibiting significant cytotoxicity. Increasing the EG chain length significantly reduced cellular entry and DC activation by NFs. Conversely, shortening the EG chain enhanced DC activation but increased toxicity and impaired water-dispersibility, resulting in low cellular uptake. These results show that the interaction of antigen-lo...Continue Reading

References

Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D A KirschnerD J Selkoe
Jan 1, 1995·Journal of Biomaterials Science. Polymer Edition·H AyhanE Pişkin
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·A LomakinD B Teplow
Jun 26, 2003·Journal of the American Chemical Society·Ming ZhengXueying Huang
Jun 3, 2004·Nature Reviews. Immunology·Seung-Yong Seong, Polly Matzinger
Jun 26, 2004·Biotechnology Letters·Satoshi YamaguchiTeruyuki Nagamune
Jun 18, 2005·International Journal of Pharmaceutics·Camilla FogedAnne Sundblad
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stephanie E A GrattonJoseph M DeSimone
Nov 7, 2008·Pharmaceutical Research·Bret D UleryBryan H Bellaire
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jai S RudraJoel H Collier
Jun 29, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ya-Ling ChiuHsing-Wen Sung
Sep 8, 2010·Angewandte Chemie·Mariusz SkwarczynskiIstvan Toth
Dec 7, 2010·Chembiochem : a European Journal of Chemical Biology·Arin GhasparianJohn A Robinson
Mar 29, 2011·Acta Biomaterialia·Maria P TorresBalaji Narasimhan
Feb 9, 2012·Journal of the American Chemical Society·Arijit GhoshJoshua E Goldberger
Feb 22, 2012·Journal of the American Chemical Society·Daniel F MoyanoVincent M Rotello
Feb 23, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Qingbin MengKeliang Liu
May 17, 2012·Journal of the American Chemical Society·Zhi-Hua HuangYan-Mei Li
May 24, 2012·International Journal of Nanomedicine·Wenxue MaBoris Minev
Feb 5, 2014·Journal of Nanobiotechnology·Li ShangGerd Ulrich Nienhaus
Mar 8, 2014·Seminars in Immunology·Daniel T FisherSharon S Evans
Jun 14, 2014·Advanced Healthcare Materials·Rebecca R PompanoAnita S Chong
Aug 16, 2014·Biomaterials·Mohammad-Ali ShahbaziHélder A Santos
Dec 30, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Qin ZengXun Sun
Jul 15, 2015·Current Opinion in Immunology·Yi Wen, Joel H Collier
Jan 1, 2014·Vaccines·Weidang LiAshlesh K Murthy
Dec 1, 2015·Small·Daniel F MoyanoVincent M Rotello
Feb 16, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eleni Maria VarypatakiWim Jiskoot
Sep 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuta YoshizakiKenji Kono
Feb 1, 2016·Chemical Science·Mariusz Skwarczynski, Istvan Toth
Sep 29, 2017·Bioconjugate Chemistry·Hamilton KakwereKatherine W Ferrara

❮ Previous
Next ❯

Citations

May 10, 2020·International Journal of Molecular Sciences·Ayae Sugawara-Narutaki, Yukiko Kamiya
Dec 18, 2020·International Journal of Molecular Sciences·Tomonori WakuNaoki Tanaka
May 22, 2021·Biomaterials Science·Vijay KumarAshwani Kumar Thakur
Aug 8, 2021·Advanced Drug Delivery Reviews·Yingyue DingQuanyin Hu

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
circular
transmission
atomic force microscopy
dynamic light scattering
flow cytometry
enzyme-linked immunosorbent assay
ELISA
Assay

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.