Effect of the mobile phase composition on the adsorption behavior of tryptophan in reversed-phase liquid chromatography

Journal of Chromatography. a
Tarab Ahmad, Georges Guiochon

Abstract

Single-component adsorption isotherm data of l-tryptophan on a C18-bonded silica column were acquired by frontal analysis (FA), with aqueous mobile phases containing 2.5, 5, and 7.5% of acetonitrile (ACN) or 7, 10, 15, and 20% of methanol (MeOH). Most of these isotherms have two inflection points and three different parts. The low and the high concentration parts exhibit langmuirian behavior. The intermediate part exhibits anti-langmuirian behavior. The inflection points shift toward higher concentrations with increasing mobile phase concentration in ACN or MeOH, which causes the differences in the isotherm profiles. The nature of the organic modifier and its concentration affect only the isotherm profile and the numerical values of its parameters, not the nature of the best model, which is the bi-Moreau model in all cases. The isotherm profiles depend on the experimental conditions because they affect the intensity of the adsorbate-adsorbate interactions. Overloaded band profiles of tryptophan were recorded with the seven mobile phase compositions. They were used to determine the best values of the isotherm coefficients by the inverse method (IM) of chromatography. There is an excellent agreement between the values of these pa...Continue Reading

References

May 18, 2001·Journal of Chromatography. a·I RustamovY V Kazakevich
Feb 25, 2003·Journal of Chromatography. a·Attila FelingerGeorges Guiochon
Mar 19, 2003·Journal of Chromatography. a·Xiaoda LiuGeorges Guiochon
Sep 17, 2003·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Aug 28, 2004·Journal of Chromatography. a·Fabrice Gritti, Georges Guiochon
Oct 27, 2004·Journal of Chromatography. a·Hyunjung KimGeorges Guiochon
Jul 1, 2005·Analytical Chemistry·Fabrice Gritti, Georges Guiochon

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