Effect of the nonreducing end of Shigella dysenteriae type 1 O-specific oligosaccharides on their immunogenicity as conjugates in mice

Proceedings of the National Academy of Sciences of the United States of America
Vince PozsgayJohn B Robbins

Abstract

Endemic and epidemic shigellosis, an acute invasive disease of the lower intestines, afflicts millions of people worldwide with an estimated one million fatalities per annum at a low infectious dose. Our approach to vaccine development against Shigella is based on the hypothesis that serum IgG antibodies to the O-specific polysaccharide (O-SP) domains of the LPS of these organisms confer protection to infection. The synthetic oligosaccharides corresponding to the tetrasaccharide repeating unit of the O-SP of Shigella dysenteriae type 1 covalently linked to human serum albumin elicited O-SP-specific IgG in mice. The antibody levels were a function of both the saccharide chain length and their loading on the protein. These synthetic saccharide conjugates elicited significantly higher levels of IgG anti O-SP than conjugates prepared with the O-SP from the bacteria. Here, we evaluated the influence of the nonreducing terminal monosaccharide on the serum antibody response. To this end, we prepared synthetic oligosaccharides comprising hexa- to tridecasaccharide fragments of the native O-SP, having one of the four monosaccharide residues that constitute the repeating unit at their termini and bound them to BSA by a single-point attac...Continue Reading

References

Jun 21, 2012·The Journal of Organic Chemistry·Vince PozsgayRachel Schneerson
Apr 2, 2010·Nature Reviews. Drug Discovery·Rena D Astronomo, Dennis R Burton
Apr 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John B RobbinsRachel Schneerson
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joanna Kubler-KielbRachel Schneerson
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·John B RobbinsMark A Miller
May 6, 2011·Glycoconjugate Journal·Tomasz LipinskiDavid R Bundle
Aug 2, 2008·Microbes and Infection·Armelle PhaliponPhilippe J Sansonetti
Feb 12, 2015·Bioconjugate Chemistry·Nina S PrasanphanichRichard D Cummings
Dec 1, 2009·Expert Review of Vaccines·Robert W Kaminski, Edwin V Oaks
Apr 24, 2014·Therapeutic Advances in Vaccines·Shai Ashkenazi, Dani Cohen
Jun 4, 2019·Human Vaccines & Immunotherapeutics·Louis-Antoine Barel, Laurence A Mulard
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Armelle PhaliponLaurence A Mulard
Aug 1, 2013·Chemical Science·Roberto AdamoGonçalo J L Bernardes
May 20, 2014·Organic & Biomolecular Chemistry·Charles GauthierLaurence A Mulard
Aug 18, 2017·Angewandte Chemie·Peter H SeebergerMartin Witzenrath
Jul 18, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cinzia ColomboLuigi Lay

Citations

Dec 1, 1971·The Journal of Clinical Endocrinology and Metabolism·J VaitukaitisG T Ross
Aug 1, 1980·The Journal of Experimental Medicine·Rachel SchneersonJohn B Robbins
Oct 24, 2001·The Journal of Organic Chemistry·Vince Pozsgay
Jan 14, 2004·Emerging Infectious Diseases·Shanta DuttaShin-ichi Yoshida
Aug 13, 2005·The Journal of Organic Chemistry·Joanna Kubler-Kielb, Vince Pozsgay
Jan 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Armelle PhaliponLaurence A Mulard
Feb 7, 2006·Carbohydrate Research·Anikó FeketeVince Pozsgay
Aug 28, 2007·Lancet·Brendan M Reilly

Related Concepts

Severe Acute Respiratory Syndrome
Galactose Measurement
Dysentery, Bacillary
Albumin Human, USP
Human Serum Albumin [EPC]
Immunogenetics
Galactose
Antibody Formation
Antibody Studies (Procedure)
Shigella

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