Effect of the oestrous cycle, pregnancy and uterine region on the responsiveness of the isolated mouse uterus to prostaglandin F(2alpha) and the thromboxane mimetic U46619

The Journal of Endocrinology
A L GriffithsDavid Woodward

Abstract

Previous studies in this laboratory have suggested that the isolated uterus from non-pregnant mice has a prostaglandin F and a thromboxane receptor population similar to that found in human myometrium. The aim of this study was to investigate any regional variation in myogenic activity ) and the and responsiveness to prostaglandin F(2alpha) (PGF(2alpha) thromboxane mimetic U46619 in the mouse uterus taken during different stages of the oestrous cycle and during pregnancy. Uterine samples from BKW mice were taken from different anatomical segments along the length of each uterine horn and set up for superfusion at 2 ml/min with Krebs solution (containing 1 microM indometacin) at 37 degrees C, and gassed with 95%O(2)/5%CO(2). Responses (area under the curve) are expressed as a percentage of the final contraction induced by hypotonic shock. Data are expressed as the means +/- s.e.m. of n=5-12 and were analysed using Student's paired t-test or two-way ANOVA with a Bonferroni post hoc test. Regional variation in myogenic activity was observed in all tissues studied except those taken during labour. These tissues displayed significantly greater myogenic activity than tissues taken at late gestation and at all stages of the oestrous c...Continue Reading

Citations

Dec 12, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Takio KitazawaTetsuro Taneike
Jan 30, 2009·Biology of Reproduction·Dan TongGerald M Kidder
Mar 1, 2012·The Journal of Pharmacology and Experimental Therapeutics·Emma S DariosStephanie W Watts
Jul 25, 2009·British Journal of Pharmacology·R L JonesD F Woodward
May 24, 2011·American Journal of Obstetrics and Gynecology·Denis J Crankshaw, John J Morrison

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