Effect of the Organization of Rhodopsin on the Association between Transducin and a Photoactivated Receptor

The Journal of Physical Chemistry. B
Samuel A Ramirez, Chad Leidy

Abstract

After photoactivation, rhodopsin (R), a G-protein-coupled receptor, rapidly activates multiple transducin G-proteins (G) in an initial amplification step of phototransduction. G-protein activation requires diffusion-mediated association with an active rhodopsin (R*) at the rod disk membrane. Different organizations of R within the membrane have been revealded by several microscopy studies, including static and freely diffusing situations. However, it is unclear how such different scenarios influence the activation rate of G proteins. Through Monte Carlo simulations, we study the association reaction between a photoactivated rhodopsin and transducin under different reported receptor organizations including (a) R monomers diffusing freely, (b) R forming static dispersed crystalline domains made of rows of dimers, and (c) R arranged in static tracks formed by two adjacent rows of dimers. A key parameter in our simulations is the probability of binding following a collision ( p). For high p, the association rate between R* and G is higher in the freely diffusive system than in the static organizations, but for low collision efficiencies, the static organizations can result in faster association rates than the mobile system. We also...Continue Reading

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Citations

Feb 17, 2021·Pflügers Archiv : European journal of physiology·Paul S-H Park
Jul 16, 2021·PLoS Computational Biology·Samuel A RamirezTimothy C Elston

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