Aug 1, 1976

Effect of the pH of culture medium on the alkalophilicity of a species of Bacillus

Archives of Microbiology
N Koyama, Y Nosoh

Abstract

The amino acid incorporation and alpha-amino-isobutyric acid (AIB) uptake of an alkalophilic Bacillus grown at pH 8.2 (the pH 8-bacteria) were much less pH dependent (less alkalophilic) than those of the organisms grown at pH 10.0 (the pH 10-bacteria), respectively. The rate of AIB uptake of the pH 10-bacteria was almost the same as that of the pH 8-bacteria, while the rate of amino acid incorporation of the pH 10-bacteria was higher than that of the pH 8-bacteria in alkaline environments. The colloidal titration with clupein showed that the amount of negative charge on the pH 10-bacteria was greater than that of the pH 8-bacteria in alkaline environments. Considerable difference in protein composition was observed between the membranes of the pH 8- and 10-bacteria while no difference was observed in phospholipid composition.

Mentioned in this Paper

Bacterial Proteins
Tissue Membrane
Uptake
Isobutyric acid
Aminoisobutyric Acids
Phospholipids
Phospholipid Measurement
Titration Method
Amino Acid [EPC]
Hydrogen-Ion Concentration

About this Paper

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