Effect of the sequences upstream from the ribosome-binding site on the yield of protein from the cloned gene for phage MS2 coat protein

Gene
R A KasteleinJ van Duin

Abstract

The translational efficiency of the coat protein gene of phage MS2 has been examined in vivo with respect to neighbouring sequences. The cloned MS2 DNA has been gradually shortened starting at the 5' or 3' terminus, and its effect on coat protein synthesis monitored. Removal of the 3'-terminal sequences had little influence. In contrast, the gradual removal of the 5'-terminal region profoundly reduces translation. Long before the ribosomal binding site (RBS) of the coat protein (CP) gene is reached, the yield of CP has dropped by one order of magnitude. Functional half-lives of the various messengers were found not to be significantly different. Available evidence indicates that the secondary structure of the RBS in native and shortened MS2 RNA is identical. We infer that important determinants for ribosome recognition lie 5' to the RBS region of the MS2 RNA coat gene.

References

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Citations

Jan 1, 1984·Molecular & General Genetics : MGG·H OhsawaC Gualerzi
Jun 24, 1985·Biochimica Et Biophysica Acta·G GrossH Blöcker
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W F JacobA E Dahlberg
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Related Concepts

DNA Sequence
Capsid
Coliphages
DNA Conformation
Ribosomes
Poly(A) Tail
RNA, Viral
Protein Biosynthesis
Viral Proteins

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