Effect of the sequences upstream from the ribosome-binding site on the yield of protein from the cloned gene for phage MS2 coat protein

R A KasteleinJ van Duin


The translational efficiency of the coat protein gene of phage MS2 has been examined in vivo with respect to neighbouring sequences. The cloned MS2 DNA has been gradually shortened starting at the 5' or 3' terminus, and its effect on coat protein synthesis monitored. Removal of the 3'-terminal sequences had little influence. In contrast, the gradual removal of the 5'-terminal region profoundly reduces translation. Long before the ribosomal binding site (RBS) of the coat protein (CP) gene is reached, the yield of CP has dropped by one order of magnitude. Functional half-lives of the various messengers were found not to be significantly different. Available evidence indicates that the secondary structure of the RBS in native and shortened MS2 RNA is identical. We infer that important determinants for ribosome recognition lie 5' to the RBS region of the MS2 RNA coat gene.


Sep 11, 1979·Nucleic Acids Research·H Donis-Keller
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A Steitz
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jan 1, 1981·Annual Review of Microbiology·L GoldG Stormo
Sep 1, 1980·European Journal of Biochemistry·C BackendorfJ van Duin


Jan 1, 1984·Molecular & General Genetics : MGG·H OhsawaC Gualerzi
Jun 24, 1985·Biochimica Et Biophysica Acta·G GrossH Blöcker
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W F JacobA E Dahlberg
Jan 12, 1987·Nucleic Acids Research·M C GanozaB G Louis
Jan 1, 1985·Antonie van Leeuwenhoek·A C AntonissenC P van Boven
Oct 12, 2000·FEBS Letters·Indulis CielensP Pumpens
Jan 7, 1994·Journal of Molecular Biology·M H de Smit, J van Duin
Sep 1, 1987·Scandinavian Journal of Immunology·P BillP A Peterson

Related Concepts

DNA Sequence
DNA Conformation
Poly(A) Tail
RNA, Viral
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