Effect of the Strawberry Genotype, Cultivation and Processing on the Fra a 1 Allergen Content

Nutrients
Elisabeth KurzeWilfried Schwab

Abstract

Birch pollen allergic patients show cross-reactivity to vegetables and fruits, including strawberries (Fragaria × ananassa). The objective of this study was to quantify the level of the Fra a 1 protein, a Bet v 1-homologous protein in strawberry fruits by a newly developed ELISA, and determine the effect of genotype, cultivation and food processing on the allergen amount. An indirect competitive ELISA using a specific polyclonal anti-Fra a 1.02 antibody was established and revealed high variability in Fra a 1 levels within 20 different genotypes ranging from 0.67 to 3.97 µg/g fresh weight. Mature fruits of red-, white- and yellow-fruited strawberry cultivars showed similar Fra a 1 concentrations. Compared to fresh strawberries, oven and solar-dried fruits contained slightly lower levels due to thermal treatment during processing. SDS-PAGE and Western blot analysis demonstrated degradation of recombinant Fra a 1.02 after prolonged (>10 min) thermal treatment at 99 °C. In conclusion, the genotype strongly determined the Fra a 1 quantity in strawberries and the color of the mature fruits does not relate to the amount of the PR10-protein. Cultivation conditions (organic and conventional farming) do not affect the Fra a 1 level, and...Continue Reading

References

Jul 11, 2000·The Journal of Allergy and Clinical Immunology·H Breiteneder, C Ebner
Jul 19, 2000·International Archives of Allergy and Immunology·K Hoffmann-Sommergruber
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·M BeslerA Paschke
Jun 23, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·F KaramlooS Vieths
May 23, 2002·Annals of the New York Academy of Sciences·Stefan ViethsBarbara Ballmer-Weber
Jun 10, 2004·Journal of Agricultural and Food Chemistry·Patrick GruberThomas Hofmann
May 3, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·L ZuidmeerR van Ree
Feb 20, 2007·Molecular Nutrition & Food Research·Emilia V VassilopoulouRonald van Ree
Jul 5, 2007·Journal of Proteome Research·Rikard AlmCecilia Emanuelsson
Oct 23, 2009·Journal of Agricultural and Food Chemistry·Anne Matthes, Michaela Schmitz-Eiberger
Dec 9, 2009·Molecular Plant·Cristina MuñozWilfried Schwab
Jan 1, 2010·The Journal of Allergy and Clinical Immunology·Scott H Sicherer, Hugh A Sampson
Apr 14, 2010·Nutrition Reviews·Arpita BasuTimothy J Lyons
Jul 20, 2011·Journal of the Science of Food and Agriculture·Sabine DöllePhilipp Franken
Oct 1, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S DölleM Worm
Dec 14, 2011·Nutrition·Francesca GiampieriMaurizio Battino
Aug 24, 2012·Bioscience Reports·Christian Seutter von LoetzenOlivia Hartl-Spiegelhauer
Apr 4, 2014·TheScientificWorldJournal·Mau SinhaTej P Singh
Mar 18, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kitty C M VerhoeckxIan Kimber
Apr 19, 2016·Journal of Agricultural and Food Chemistry·Katrin Franz-OberdorfWilfried G Schwab
May 14, 2016·Journal of Agricultural and Food Chemistry·Sadia AfrinMaurizio Battino
Sep 7, 2017·Food Research International·Katrin Franz-OberdorfWilfried Schwab
Nov 22, 2017·Applied Microbiology and Biotechnology·David J WurmOliver Spadiut

❮ Previous
Next ❯

Citations

Jul 16, 2020·Nutrients·Abigail E CullenJavier Fernandez andGloria Salazar
Jun 6, 2020·Scientific Reports·Emilia RomerWilfried Schwab
Jul 29, 2021·The Plant Journal : for Cell and Molecular Biology·Mehran Dastmalchi
Nov 28, 2019·Journal of Agricultural and Food Chemistry·Begoña Orozco-NavarreteCatharina Merchante

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
thermal treatment

Software Mentioned

MARS
- Plot
R
R Foundation for Statistical Computing , R

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.