Effect of the timing of the first cleavage on the developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells

Theriogenology
T KobayashiYukio Tsunoda

Abstract

The present study examined whether the timing of the first cleavage has an effect on the in vitro and in vivo developmental potential of nuclear-transferred mouse oocytes receiving embryonic stem cells. First, the timing of the first cleavage and the developmental potential of nuclear-transferred oocytes were examined every hour from 12 to 24 h after the start of culture and compared with in vitro-fertilized oocytes. The developmental potential of in vitro-fertilized oocytes decreased gradually according to the time required for cleavage (84% (32/38) for 15 h to 50% (1/2) for 20 h), but intermediate-cleaved (15-16 h) nuclear-transferred oocytes had a higher potential to develop into blastocysts (55% (17/31) to 67% (45/67) versus 0-43% (6/14)]. Second the nuclear-transferred oocytes were divided into three groups according to the timing of the first cleavage; each group was cultured to blastocysts in vitro, and then transferred to recipients. The potential of intermediate-cleaved oocytes (15-16 h) to develop into blastocysts was significantly higher than fast-cleaved (before 15 h) and slow-cleaved (after 16 h) oocytes (65, 46, and 37%). The proportion of fetuses on Day 10.5 of pregnancy was highest in the intermediate-cleaved gr...Continue Reading

References

Jul 1, 1975·Journal of Reproduction and Fertility·Y Tsunoda, M C Chang
Feb 2, 2000·Nature Genetics·W M RideoutR Jaenisch
Nov 4, 2000·Journal of Reproduction and Fertility·Y KatoY Tsunoda
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·K EgganR Jaenisch
Jul 7, 2001·Science·D HumpherysR Jaenisch
Feb 28, 2003·Biology of Reproduction·Young Gie ChungKeith E Latham
Mar 19, 2003·Differentiation; Research in Biological Diversity·Wei ShiEckhard Wolf

❮ Previous
Next ❯

Citations

Feb 11, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Thanh Quang Dang-NguyenBui Xuan Nguyen
Oct 1, 2018·Journal of Cellular Biochemistry·Vahideh AssadollahiOzra Allahvaisi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.