Effect of the uvrD mutation on excision repair.

Journal of Bacteriology
N B Kuemmerle, W E Masker

Abstract

A pair of related Escherichia coli K-12 strains, one of which contains the uvrD101 mutation, were constructed and compared for ability to perform various steps in the excision repair of deoxyribonucleic acid damage inflicted by ultraviolet radiation. The results of this study indicated: (i) ultraviolet sensitivity in the uvrD101 mutant was greater than that of wild type but less than that measured in an incision-deficient uvrA mutant; (ii) host cell reactivation paralleled the survival data; (iii) postirradiation deoxyribonucleic acid degradation was virtually identical in the two strains; (iv) incision, presumably at the sites of pyrimidine dimers, proceeded normally in the uvrD101 strain; (v) excision of pyrimidine dimers was markedly reduced in both rate and extent in the uvrD101 mutant; (vi) the amount of repair resynthesis was the same in both strains, and there was no evidence of abnormally long repair patches in the uvrD mutant; and (vii) rejoining of incision breaks was slow and incomplete in the uvrD strain. These data suggest that the ultraviolet sensitivity conferred by the uvrD mutation arises from inefficient removal of pyrimidine dimers or from failure to close incision breaks. The data are compatible with the not...Continue Reading

References

Aug 18, 1976·Biochimica Et Biophysica Acta·W E Masker
Sep 1, 1977·Journal of Virology·N B Kuemmerle, W E Masker
Oct 24, 1977·Molecular & General Genetics : MGG·R H Rothman, A J Clark
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·E Seeberg
Jan 1, 1979·Annual Review of Biochemistry·P C HanawaltC A Smith
Nov 1, 1976·Journal of Molecular Biology·G PawlE C Friedberg
Dec 1, 1976·Bacteriological Reviews·E M Witkin
Jan 1, 1975·Annual Review of Biochemistry·L GrossmanI Mahler
May 1, 1966·Journal of Molecular Biology·R B Setlow, W L Carrier
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·P K Cooper, P C Hanawalt
Jun 14, 1972·Journal of Molecular Biology·P K Cooper, P C Hanawalt
Jan 1, 1972·Annual Review of Biophysics and Bioengineering·R B Setlow, J K Setlow
Jul 2, 1973·Biochemical and Biophysical Research Communications·B I SinzinisA A Saenko
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E A WaldsteinR Ben-Ishai
Jan 1, 1968·Annual Review of Biochemistry·P Howard-Flanders
May 3, 1968·Molecular & General Genetics : MGG·H OgawaJ Tomizawa
Nov 1, 1969·Virology·F W Studier
Apr 8, 1970·Biochemical and Biophysical Research Communications·L Kanner, P Hanawalt
Jan 1, 1970·Molecular & General Genetics : MGG·H Ogawa
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R E Moses, C C Richardson
Feb 26, 1960·Science·L GORINI, H KAUFMAN

❮ Previous
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Citations

Sep 24, 1990·European Journal of Biochemistry·R WesselH Hoffmann-Berling
Jul 21, 2006·Journal of Bacteriology·Josephine Kang, Martin J Blaser
Jan 1, 1983·Molecular & General Genetics : MGG·I D HicksonP T Emmerson
Dec 30, 2015·Science China. Life Sciences·David K Orren
Oct 28, 2016·Photochemistry and Photobiology·Michael G Kemp, Jinchuan Hu
Feb 9, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ogun AdebaliChristopher P Selby
Dec 1, 1980·Photochemistry and Photobiology·R H Rothman
Nov 20, 2016·Photochemistry and Photobiology·Christopher P Selby
Sep 24, 1990·European Journal of Biochemistry·P Georgi-Geisberger, H Hoffmann-Berling
Aug 1, 1981·Journal of Bacteriology·N KuemmerleW Masker
Jan 1, 1993·Journal of Bacteriology·B K Washburn, S R Kushner
Mar 1, 1981·Microbiological Reviews·C Bernstein
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·H L RocchettaJ S Lam
Jan 1, 1989·Pharmacology & Therapeutics·K Drlica, S Coughlin
Aug 1, 1987·Journal of Bacteriology·H M ArthurP T Emmerson
Sep 18, 1997·Microbiology and Molecular Biology Reviews : MMBR·K Drlica, X Zhao
Mar 1, 1990·Microbiological Reviews·B Van Houten
Nov 1, 1983·Gene·H M Arthur, P B Eastlake

❮ Previous
Next ❯

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