PMID: 8581518Aug 1, 1995Paper

Effect of theophylline on beta-adrenergic receptor density and cAMP content in bovine aortic smooth muscle cells

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
K GrafG Kunkel

Abstract

Activation of vascular beta-adrenergic receptors prevents an increase in vascular permeability caused by free radicals or inflammatory peptides. Methylxanthines seem to have similar protective effects on vascular endothelium. In the present study we investigated the effect of theophylline on the beta-adrenergic receptor expression and cAMP concentrations in cultured endothelial and smooth muscle cells from bovine aorta. Comparable values for beta-receptor density and binding affinity were detected in both cell types. Isoproterenol induced significant downregulation of beta-receptors in endothelial (BAEC: -60.5%) and smooth muscle cells (BASMC: -52.5%; P < 0.01). Incubation of endothelial cells with theophylline (4 micrograms/ml and and 40 micrograms/ml) for 24 hours did not affect beta-receptor expression, whereas in smooth muscle cells the beta-receptor density was reduced for -31.5% and -28.7%, respectively. In endothelial cells a transient effect on cAMP concentrations was observed after stimulation with isoproterenol (1 microM), but no effect was found in theophylline treated endothelial cells. Stimulation of intact smooth muscle cells with isoproterenol and theophylline (4 micrograms/ml and 40 micrograms/ml) resulted in a ...Continue Reading

References

Jan 1, 1979·Physiological Reviews·J Chamley-CampbellR Ross
Jan 1, 1979·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·T FoyT R Harris
May 16, 1990·Biochemical and Biophysical Research Communications·I Mills, H Gewirtz
Sep 1, 1988·Journal of Applied Physiology·R E HowellE M Levine
Jan 1, 1986·Journal of Applied Physiology·F L MinnearA B Malik
Apr 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·S F SteinbergJ P Bilezikian
Sep 16, 1982·Biochemical and Biophysical Research Communications·M B Anand-SrivastavaJ Genest
Apr 1, 1993·The Journal of Clinical Investigation·N SuttorpC Schudt

❮ Previous
Next ❯

Citations

May 14, 2020·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Periklis KatopodisKonstantinos P Katopodis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Related Papers

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
E LundgrenT K Borg
The Journal of Immunology : Official Journal of the American Association of Immunologists
A SevernF Y Liew
© 2021 Meta ULC. All rights reserved