Effect of thiocyanate on AMPA receptor mediated responses in excised patches and hippocampal slices

Neuroscience
A AraiG Lynch

Abstract

The binding affinity of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors for [3H]AMPA is increased 10-30-fold by the chaotropic anion thiocyanate. The present experiments tested if thiocyanate alters AMPA receptor mediated current fluxes and if any such effects are reflected in the waveform of synaptic responses. Currents were measured after a step application of glutamate or AMPA to patches excised from pyramidal cells of hippocampal slice cultures. Application of 1 mM AMPA produced responses with an average peak amplitude of 86 pA at -50 mV and a 10-90% rise time of 1.7 +/- 0.1 ms; the responses desensitized to a steady-state level below 10% of the peak current with a time constant of 11.1 +/- 0.7 ms. Glutamate in presence of D-amino-phosphonopentanoate produced similar responses which were inhibited by 6-cyano-7-nitro-quinoxaline-dione and enhanced by aniracetam or cyclothiazide and thus are characteristic for AMPA receptors. Thiocyanate accelerated the decay of AMPA responses two-fold and reduced the peak current by 30-50% with an EC50 of 3.2 mM which is comparable to its EC50 for enhancing binding. Effects on the desensitization of glutamate induced responses were much smaller and only evident at t...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Shahi, M Baudry
Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Dec 1, 1991·Synapse·J Ambros-IngersonG Lynch
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G MassicotteM Baudry
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L Mayer, L Vyklicky
Aug 1, 1989·Neuron·L O Trussell, G D Fischbach
Feb 28, 1985·Neuroscience Letters·T Honoré, M Nielsen
Feb 3, 1987·Brain Research·R W OlsenC R Houser
Nov 22, 1988·European Journal of Pharmacology·E O NielsenA B Young
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
Jun 1, 1994·Neurochemical Research·R A HallG Lynch
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Ambros-Ingerson, G Lynch
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S MarenR F Thompson

❮ Previous
Next ❯

Citations

Jan 15, 1999·Molecular Neurobiology·K K Dev, J M Henley
May 4, 2005·Molecular and Cellular Neurosciences·Erika SuzukiAmy C Arai
Jun 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Marialaura AmadioAlessia Pascale
Nov 7, 2002·Brain Research·Bin LinGary Lynch
Dec 16, 1998·European Journal of Pharmacology·B S NielsenD S Pickering
Apr 9, 2001·Environmental Toxicology and Pharmacology·H -H. ChenI K. Ho
Jul 16, 2004·Journal of Neurotrauma·Paulette B GoforthLeslie S Satin
Jan 28, 2005·The European Journal of Neuroscience·Francesco FornaiKaren Gale
Dec 20, 2014·Annals of Neurology·Olusegun Steven A Oluwole
Aug 26, 1999·Drug Metabolism Reviews·P S Spencer
Mar 21, 2019·American Journal of Physiology. Cell Physiology·Xu CaoBret A Hughes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.