Effect of titanium surface topography on macrophage activation and secretion of proinflammatory cytokines and chemokines

Journal of Biomedical Materials Research. Part a
Ali K RefaiJ Douglas Waterfield

Abstract

The macrophage has a major role in normal wound healing and the reparative process around implants. Murine macrophage-like cells RAW 264.7 were used to investigate the effect of titanium surfaces on macrophage activation and secretion of proinflammatory cytokines [interleukin (IL)-1 beta, IL-6, and tumor necrosis factor (TNF)-alpha] and chemokines (monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 alpha). Four topographies were used: those produced by mechanically polishing, coarse sand blasting, acid etching, and sandblasting and acid etching (SLA). Macrophages were plated on the four titanium surfaces at a population density of 5 x 10(5) cells/mL/well. Tissue culture plastic and tissue culture plastic plus lipopolysaccharide (LPS) served as negative and positive control, respectively. In addition, all surfaces were tested for their effects on macrophages in the presence of LPS. Supernatants were collected for assays after 6, 24, and 48 h and the numbers of macrophages attached to the surfaces were quantified using the DAPI (4,6-di-amidino-2-phenylindole) assay. Cytokine and chemokine levels were measured with sandwich enzyme-linked immunosorbent assays. Statistical comparison between the surfaces and th...Continue Reading

References

Dec 1, 1990·The Journal of Trauma·T K Hunt
Jan 1, 1988·Biomaterials·N P ZiatsJ M Anderson
Apr 1, 1984·Journal of Biomedical Materials Research·T N Salthouse
Aug 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·E J Kovacs, L A DiPietro
Jun 1, 1995·Cell Biology International·B Wójciak-StothardC Wilkinson
Sep 1, 1995·The Journal of Bone and Joint Surgery. American Volume·J E FeighanS Stevenson
Feb 1, 1995·Current Opinion in Immunology·S GordonA Doyle
Jan 1, 1994·Journal of Biomedical Materials Research·A S ShanbhagT T Glant
Dec 1, 1993·Journal of Cellular Biochemistry·G R Mundy
Sep 27, 1993·Journal of Immunological Methods·L ShapiraW A Soskolne
Nov 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Gordon
Oct 1, 1995·Journal of Biomedical Materials Research·K GotfredsenE Hjørting-Hansen
Dec 1, 1995·Journal of Biomedical Materials Research·M WongE Hunziker
Sep 1, 1996·Journal of Biomedical Materials Research·K KieswetterB D Boyan
Apr 1, 1997·Acta Orthopaedica Scandinavica·A FrostO Ljunggren
May 16, 1998·The Journal of Clinical Investigation·L A DiPietroR M Strieter
Dec 16, 1998·Research in Immunology·R van Furth
Mar 18, 1999·Journal of Biomechanical Engineering·D M Brunette, B Chehroudi
Feb 1, 1988·Journal of Dental Research·B S PearsonD Moskowicz
Dec 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R M GallucciM I Luster
Feb 13, 2001·Clinical Oral Implants Research·J Y Park, J E Davies
Feb 13, 2001·Clinical Oral Implants Research·M ScacchiA R Schär
Mar 30, 2001·Advances in Dental Research·D M Brunette
Aug 4, 2001·The American Journal of Pathology·Q E LowL A DiPietro
Jan 5, 2002·Journal of Biomedical Materials Research·Toshihiro AkisueYuichi Mochida
May 15, 2002·Clinical Oral Implants Research·W Aubrey SoskolneLior Shapira
Jan 30, 2003·Journal of Leukocyte Biology·David M Mosser

❮ Previous
Next ❯

Citations

Feb 27, 2007·Journal of Materials Science. Materials in Medicine·R WillumeitF Feyerabend
Jun 15, 2007·Journal of Materials Science. Materials in Medicine·Peter H PennekampClayton N Kraft
Sep 17, 2009·Journal of Materials Science. Materials in Medicine·D SpillerG Soldani
Aug 1, 2009·Journal of Materials Science. Materials in Medicine·Masayuki TairaYoshima Araki
Jun 26, 2009·Journal of the American Chemical Society·Karen L ChristmanHeather D Maynard
Aug 19, 2008·Biochemistry. Biokhimii︠a︡·T G Moizhess
Feb 16, 2008·Journal of Biomedicine & Biotechnology·M JägerR Krauspe
Jul 18, 2012·Nanoscale Research Letters·Mohammed MohiuddinGuewha Steven Huang
Jun 29, 2012·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Feng ZhangGeng-sheng Shi
May 9, 2014·Annals of Biomedical Engineering·Debanti SenguptaSong Li
Nov 20, 2013·Annals of Biomedical Engineering·Eric M SussmanBuddy D Ratner
Apr 19, 2013·Journal of Biomedical Materials Research. Part a·Mohammed A AlfarsiSaso Ivanovski
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·Laura SaldañaNuria Vilaboa
Jan 19, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mario Moisés AlvarezAli Khademhosseini
Jan 7, 2016·Biomaterials·Richard J Miron, Dieter D Bosshardt
Nov 26, 2015·ACS Applied Materials & Interfaces·Thuy U LuuWendy Liu
Jun 28, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Edwin LamersJohn A Jansen
Dec 25, 2007·Biomaterials·Fabio VariolaAntonio Nanci
Dec 29, 2007·Seminars in Immunology·James M AndersonDavid T Chang
Aug 27, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eric R MolinaAntonios G Mikos
Oct 30, 2013·Journal of Biomedical Materials Research. Part a·Lu HuangWei He
Jul 23, 2008·Journal of Biomedical Materials Research. Part a·Frank FeyerabendJoachim Rösler
Nov 15, 2006·Clinical Implant Dentistry and Related Research·Anna GöranssonAnn Wennerberg
Feb 21, 2008·Journal of Biomedical Materials Research. Part a·T A SchildhauerM Köller
May 16, 2007·Clinical Oral Implants Research·Mirjam M FürstG Rutger Persson
Nov 26, 2010·Journal of Biomedical Materials Research. Part a·Angela M B ColliePatrick S Stayton
Aug 1, 2012·Journal of Biomedical Materials Research. Part a·Hyun-Su LeeDavid M Eckmann
Feb 22, 2013·Journal of Biomedical Materials Research. Part a·Salem GhrebiDonald M Brunette
Feb 22, 2013·Journal of Biomedical Materials Research. Part a·Katrin A BarthDonald M Brunette
Nov 19, 2011·Clinical Oral Implants Research·Stephen HamletSaso Ivanovski
Mar 29, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anna ArvidssonVictoria Franke Stenport
Sep 9, 2010·Journal of Biomedical Materials Research. Part a·J Douglas WaterfieldDonald M Brunette
Apr 12, 2013·Clinical Implant Dentistry and Related Research·Nikola SaulacicDaniel Buser
Mar 26, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Rima M WazenAntonio Nanci
Jul 18, 2009·Journal of Biomedical Materials Research. Part a·Babak ChehroudiDonald Maxwell Brunette
Dec 16, 2006·Analytical Biochemistry·Sung Bae KimYoshio Umezawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.