Effect of Titanium Surfaces on the Osteogenic Differentiation of Human Adipose-Derived Stem Cells

The International Journal of Oral & Maxillofacial Implants
Diogo Godoy ZanicottiDawn Elizabeth Coates

Abstract

To investigate whether human adipose-derived stem cells will form a functional bone-like matrix on titanium substrates. The behavior of human adipose-derived stem cells was examined when grown in either serum-free, xeno-free stem cell growth medium or osteogenic differentiation medium and cultured on either machined titanium (MTi) or on roughened alumina-blasted titanium (ABTi) discs. Cellular proliferation, extracellular mineralized matrix production, osteogenic-related protein production (RUNX2 and osteocalcin), and gene expression for pluripotency and self-renewal (TERT and OCT4) and osteogenic-related (MSX2, RUNX2, and BGLAP) genes were performed. Human adipose-derived stem cells in serum-free medium (hADSC) proliferated at a higher rate compared with osteogenically differentiated cells (hOS-ADSC); however, the osteogenically committed cells produced more mineralized matrix on the titanium surfaces compared with either tissue culture plastic or the undifferentiated cells. The immunofluorescence analysis showed that human adipose-derived stem cells cultured in serum-free medium and osteogenic differentiation medium produced RUNX2 on both the machined titanium surface and on the alumina-blasted titanium surface after 7 days i...Continue Reading

Citations

Jan 28, 2021·Clinical Oral Implants Research·Jefferson O AbariciaRene Olivares-Navarrete
Jun 18, 2020·Dental Materials : Official Publication of the Academy of Dental Materials·Franziska EhlickeJan Hansmann

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