Effect of tocotrienols on the growth of a human breast cancer cell line in culture

Lipids
Kalanithi NesaretnamK K Carroll

Abstract

The tocotrienol-rich fraction (TRF) of palm oil consists of tocotrienols and some alpha-tocopherol (alpha-T). Tocotrienols are a form of vitamin E having an unsaturated side-chain, rather than the saturated side-chain of the more common tocopherols. Because palm oil has been shown not to promote chemically-induced mammary carcinogenesis, we tested effects of TRF and alpha-T on the proliferation, growth, and plating efficiency (PE) of the MDA-MB-435 estrogen-receptor-negative human breast cancer cells. TRF inhibited the proliferation of these cells with a concentration required to inhibit cell proliferation by 50% of 180 microgram/mL whereas alpha-T had no effect at concentrations up to 1000 microgram/mL as measured by incorporation of [3H]thymidine. The effects of TRF and alpha-T also were tested in longer-term growth experiments, using concentrations of 180 and 500 microgram/mL. We found that TRF inhibited the growth of these cells by 50%, whereas alpha-T did not. Their effect on the ability of these cells to form colonies also was studied, and it was found that TRF inhibited PE, whereas alpha T had no effect. These results suggest that the inhibition is due to the presence of tocotrienols in TRF rather than alpha T.

References

Apr 1, 1991·The American Journal of Clinical Nutrition·W Z NgahK A Kadir
Apr 1, 1991·The American Journal of Clinical Nutrition·M N GouldC E Elson
Apr 1, 1991·The American Journal of Clinical Nutrition·L Packer
May 1, 1989·Chemical & Pharmaceutical Bulletin·K KomiyamaI Umezawa
Oct 16, 1989·Biochemical and Biophysical Research Communications·D P Rose, J M Connolly
Jan 1, 1988·American Journal of Epidemiology·P KnektL Teppo
Jun 1, 1988·International Journal of Epidemiology·P Knekt

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Citations

May 24, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Alexander MeyenbergBeatrice E Frueh
Apr 13, 2011·Genes & Nutrition·Ramaswamy KannappanBharat B Aggarwal
Apr 26, 2011·Genes & Nutrition·Kalanithi NesaretnamKanga Rani Selvaduray
Sep 10, 1999·Clinical Biochemistry·A TheriaultK Adeli
Feb 9, 2000·Progress in Lipid Research·N Guthrie, K K Carroll
Jul 31, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·C K B Ferrari, E A F S Torres
Feb 9, 2002·The Journal of Nutritional Biochemistry·Dawn C. Schwenke
Aug 30, 2000·Proceedings of the Society for Experimental Biology and Medicine·B S McIntyreP W Sylvester
Apr 18, 2009·Nutrition and Cancer·Wong Weng-YewKalanithi Nesaretnam
Sep 18, 2010·Journal of the American College of Nutrition·Chandan K SenSavita Khanna
Jun 15, 2006·Antioxidants & Redox Signaling·Pramod KhoslaChandan K Sen
Dec 3, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Kanga Rani SelvadurayKalanithi Nesaretnam
Sep 15, 2009·Carcinogenesis·Jihyeung JuChung S Yang
Feb 18, 2012·Journal of Chromatographic Science·Ng Mei Han, Choo Yuen May
Jan 7, 2010·BMC Cancer·Sitti Rahma Abdul HafidKalanithi Nesaretnam
Jan 15, 2014·Nutrition & Metabolism·Ju-Yen FuKim-Tiu Teng
Aug 13, 2003·Bioscience, Biotechnology, and Biochemistry·Hitoshi InokuchiTeruo Miyazawa
Apr 25, 2007·Critical Reviews in Food Science and Nutrition·Wilailuk ChaiyasitEric A Decker
Feb 15, 2013·International Journal of Food Sciences and Nutrition·Elena Fattore, Roberto Fanelli
Mar 26, 2016·Journal of Clinical Biochemistry and Nutrition·Nozomi KaneaiShiro Urano
Feb 5, 2011·The Journal of Nutritional Biochemistry·Yang LiJia-Ren Liu
Sep 28, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Siti Aisyah Abd GhafarMaznah Ismail
Dec 17, 2008·The Journal of Nutritional Biochemistry·Teruo MiyazawaKiyotaka Nakagawa
May 28, 2008·Cancer Letters·Kalanithi Nesaretnam
May 18, 2007·Molecular Aspects of Medicine·Chandan K SenSashwati Roy
Mar 9, 2005·Annals of the New York Academy of Sciences·Kalanithi NesaretnamFabio Virgili
Mar 9, 2005·Annals of the New York Academy of Sciences·Chandan K SenSashwati Roy
May 31, 2008·International Journal of Cancer. Journal International Du Cancer·Constantina ConstantinouAndreas I Constantinou
Jan 13, 2011·The Plant Journal : for Cell and Molecular Biology·Wenyu YangEdgar B Cahoon
Jul 10, 2010·International Journal of Cancer. Journal International Du Cancer·Sze Ue LukMing-Tat Ling

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