Effect of total flavonoids of Chrysanthemum indicum on the apoptosis of synoviocytes in joint of adjuvant arthritis rats

The American Journal of Chinese Medicine
Xiao-Yu ChenRong Hu

Abstract

Chrysanthemum is a traditional Chinese medicine used in China to treat inflammatory diseases. The total flavonoids Chrysanthemum indicum (TFC) were extracted from the dried bud of Chrysanthemum indicum. Our previous study had demonstrated that TFC was a new class of effective anti-inflammation, analgesia and immunoloregulation agents. In this study, we established an adjuvant arthritis (AA) model by injection of Freund's Complete Adjuvant (FCA) to investigate the effect of TFC on the apoptosis of synoviocytes in AA Rats. Synoviocytes isolated from knee joint of rats were treated with different doses of TFC in vitro. Synoviocytes proliferation was measured by MTT assay, and DNA fragmentations were evaluated on agarose gel electrophoresis. The levels of caspase-3 cleaved fragments were analyzed by Western blot. The annexin V stain assay was used to explore the inhibition of caspase-3 on the amelioration of synoviocytes apoptosis. The results showed that TFC inhibited the proliferation of synoviocytes. Electrophoresis showed higher ladders of DNA bands in the TFC group. Cleaved fragments of caspase-3 were increased significantly. Furthermore, the apoptotic synoviocytes were markedly decreased by the caspase-3 specific inhibitor. T...Continue Reading

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Citations

Sep 25, 2009·World Journal of Gastroenterology : WJG·Zong-Fang LiXian-Ming Xia
Jan 15, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Shivaprasad H VenkateshaKamal D Moudgil
Jan 12, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·In-Su KimDong-Kug Choi
Sep 25, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Yajing SunQinglong Guo
May 27, 2010·The American Journal of Chinese Medicine·Jing LiZuo-Hui Shao
Jan 5, 2018·The American Journal of Chinese Medicine·Sarmila NepaliYoung-Mi Lee
Aug 13, 2013·Experimental and Therapeutic Medicine·Xiao-Yu ChenYu-Hua Sun

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis