Effect of transfected interleukin-6 in non-tumorigenic and tumorigenic rat urothelial cell lines

International Journal of Cancer. Journal International Du Cancer
M Okamoto, R Oyasu

Abstract

It has been suggested that interleukin (IL)-6 plays a significant role in bladder carcinomas. We conducted the present experiment to determine whether over-expressed IL-6 enhanced the tumorigenicity of a weakly tumorigenic rat bladder carcinoma, and whether it was sufficient to induce a tumorigenic phenotype in a non-tumorigenic, anchorage-dependent rat urothelial cell line, MYP3. P3M6-10 and P3M6-12 (anchorage-independent but non-tumorigenic) and MYP3T6 (anchorage-independent and tumorigenic) were isolated from MYP3 after treatment with MNU in vitro. None of these clones produced IL-6. The cells were transfected with an expression vector containing human IL-6 cDNA. The transfectants secreted a large amount of IL-6. In MYP3T6, over-expression of IL-6 enhanced tumorigenicity markedly in nude mice, as evidenced by acceleration of the growth rate in vivo as well as of anchorage-dependent and -independent growth. In P3M6-10 and P3M6-12, the introduced IL-6 cDNA markedly enhanced their growth potential, both on a plastic surface and in soft agar, but did not induce a tumorigenic potential. In MYP3, introduced IL-6 weakly enhanced their growth on a plastic surface, but caused neither tumorigenesis in nude mice nor colony formation in...Continue Reading

References

Sep 1, 1992·The Journal of Urology·T SeguchiA Okuyama
Nov 20, 1991·Journal of the National Cancer Institute·J TakenawaO Yoshida

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Citations

Jan 1, 1997·Mediators of Inflammation·S Ben-Efraim
Jun 29, 2005·International Journal of Oral and Maxillofacial Surgery·T ShibaharaH Noma
Oct 9, 2013·Expert Opinion on Therapeutic Targets·Shili Xu, Nouri Neamati
Jun 2, 2012·Cancer Treatment Reviews·Yuqi GuoZhan Zhang
Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·M OkamotoR Oyasu

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