Effect of transforming growth factor beta-1 on the cholesterol side-chain cleavage system in the adrenal gland of sheep fetuses and newborns

Endocrinology
E Naaman-ReperantP Durand

Abstract

The present work examined the effect of transforming growth factor-beta1 (TGFbeta1) on the cholesterol side-chain cleavage system of sheep fetal and neonatal adrenal glands. Freshly isolated fetal adrenal cells produced 3-fold less pregnenolone from 22R- hydroxycholesterol than neonatal cells. Also, the relative amounts of immunoreactive cytochrome P450 side-chain cleavage (P450scc), adrenodoxin, and adrenodoxin reductase were 1.5- to 2-fold lower in mitochondria from fetal than neonatal cells. However, during culture under control conditions, the cholesterol side-chain cleavage activity and the amounts of P450scc, adrenodoxin, and adrenodoxin reductase of fetal cells increased after 48 h to reach neonatal values. A 5-day treatment with TGFbeta1 (1ng/ml) decreased significantly both the cholesterol side-chain cleavage activity and the amounts of immunoreactive P450scc, adrenodoxin, and adrenodoxin reductase in sheep fetal and neonatal adrenal cells in culture. Immunoreactive TGFbeta1- like material was present in freshly isolated adrenal cells from both fetuses and newborn lambs. After 2 days of culture, the amount of TGFbeta1-like protein was 2-fold lower than in freshly isolated fetal cells. No change was observed for neonata...Continue Reading

Citations

Jun 6, 2009·Experimental Biology and Medicine·Holly A Lavoie, Steven R King
Jan 15, 2015·Current Opinion in Nephrology and Hypertension·Kota MatsukiMasao Kakoki
Jan 1, 2016·American Journal of Physiology. Renal Physiology·Albert S ChangMasao Kakoki

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