Effect of transport at ambient temperature on detection and isolation of Vibrio cholerae from environmental samples

Applied and Environmental Microbiology
Munirul AlamRita R Colwell

Abstract

It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting environmental samples at the ambient air temperature on isolation and enumeration of V. cholerae. For water and plankton samples held at ambient temperatures ranging from 31 degrees C to 35 degrees C for 20 h, the total counts did not increase significantly but the number of culturable V. cholerae increased significantly compared to samples processed within 1 h of collection, as measured by culture, acridine orange direct count, direct fluorescent-antibody-direct viable count (DFA-DVC), and multiplex PCR analyses. For total coliform counts, total bacterial counts, and DFA-DVC counts, the numbers did not increase significantly, but the culturable plate counts for V. cholerae increased significantly after samples were held at the ambient temperature during transport to the laboratory for analysis. An increase in the recovery of V. cholerae O1 and improved detection of V. cholerae O1 rfb and ctxA also occurred when samples ...Continue Reading

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Citations

Jul 19, 2011·Applied and Environmental Microbiology·Guillaume Constantin de MagnyRita R Colwell
Feb 2, 2010·The Journal of General and Applied Microbiology·Cindy Shuan Ju TehThandavarayan Ramamurthy
Apr 23, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Cindy Shuan Ju TehKwai Lin Thong
Nov 12, 2014·The American Journal of Tropical Medicine and Hygiene·Amy M KahlerVincent R Hill
Sep 5, 2008·Current Protocols in Microbiology·Anwar HuqG Balakrish Nair
Aug 10, 2012·Current Protocols in Microbiology·Anwar HuqRita R Colwell
Jul 21, 2017·Microbes and Environments·Léa GirardJulia Baudart

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