Effect of Transportation on Cultured Limbal Epithelial Sheets for Worldwide Treatment of Limbal Stem Cell Deficiency

Scientific Reports
O A UtheimT P Utheim

Abstract

Limbal stem cell deficiency can be treated with transplantation of cultured human limbal epithelial cells (LEC). It can be advantageous to produce LEC in centralized labs and thereafter ship them to eye clinics. The present study used transport simulations of LEC to determine if vigorous shaking during transport altered the viability, morphology and phenotype during a 4 day-long storage of LEC with a previously described serum-free storage method. Inserts with LEC cultured on amniotic membranes were sutured to caps inside air-tight containers with generous amounts of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered minimal essential medium (MEM). The containers were distributed among the following testing conditions: 6 hours with full containers, 36 hours with full containers, 36 hours with container three quarters full of medium, and 36 hours with container full of medium containing a shear-protecting agent (Pluronic-F68). Compared to stored, but non-transported controls, no statistically significant changes in viability and immunohistochemical staining were observed. The epithelial sheets remained intact. However, an air-liquid interface in the containers reduced the number of desmosomes and hemi-desmosomes...Continue Reading

References

Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Mar 1, 1997·American Journal of Ophthalmology·S H Lee, S C Tseng
Mar 27, 2002·The British Journal of Ophthalmology·D MellerS C G Tseng
Apr 19, 2005·The British Journal of Ophthalmology·H S DuaA Joseph
Jun 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Enzo Di IorioMichele De Luca
Nov 25, 2006·The British Journal of Ophthalmology·Tor Paaske UtheimBjørn Nicolaissen
May 1, 2007·Regenerative Medicine·Julie T DanielsShankar Seetharaman
Jun 15, 2007·The Journal of Cell Biology·Vanessa BarbaroMichele De Luca
May 28, 2010·Journal of Cellular Physiology·Sajjad AhmadUla Jurkunas
Jun 25, 2010·The New England Journal of Medicine·Paolo RamaGraziella Pellegrini
May 22, 2013·Methods in Molecular Biology·Tor Paaske UtheimSten Ræder
Sep 21, 2013·Tissue Engineering. Part C, Methods·Yoshinori OieKohji Nishida
Mar 10, 2015·Nature Biotechnology·Elie Dolgin

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transmission electron microscopy
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