Effect of trehalose on W7FW14F apomyoglobin and insulin fibrillization: new insight into inhibition activity

Biochemistry
Silvia VilasiIvana Sirangelo

Abstract

Trehalose, a disaccharide present in many nonmammalian species, protects cells against various environmental stresses. Trehalose has recently been shown to decrease aggregate formation and toxicity in cell models and to alleviate amyloid-induced diseases. The aim of our study was to use two amyloid-forming proteins, i.e., W7FW14F apomyoglobin and insulin, as model systems to elucidate the molecular mechanism by which trehalose affects the amyloid aggregation process and to investigate further its therapeutic potential. Protein aggregation was examined by far-UV circular dichroism, UV absorption, thioflavin T fluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, atomic force microscopy, and Fourier transform infrared spectroscopy. Cell viability was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay. We found that trehalose does not inhibit protein aggregation but acts at different stages of the fibrillization process depending on the protein model used. In fact, trehalose dose-dependently inhibited fibril formation in the W7FW14F apomyoglobin model and increased the lag phase in the insulin model. In both cases, trehalose caused accumulation of toxic oligomeric specie...Continue Reading

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Citations

Dec 11, 2013·PloS One·Clara IannuzziIvana Sirangelo
Oct 29, 2014·Journal of Molecular Modeling·Daixi LiBaolin Liu
Sep 3, 2013·Food & Function·Atefeh RabieeMohsen Nemat-Gorgani
May 2, 2012·International Journal of Pharmaceutics·Wei WangSandeep Nema
Jul 24, 2014·Journal of Cellular Biochemistry·Ivana SirangeloMaria Luisa Balestrieri
Nov 1, 2015·Biochimica Et Biophysica Acta·Clara IannuzziIvana Sirangelo
Nov 22, 2012·Journal of Cellular Physiology·Ivana SirangeloMaria Luisa Balestrieri
Apr 8, 2015·Journal of Cellular Physiology·Clara IannuzziIvana Sirangelo
Oct 24, 2012·The International Journal of Biochemistry & Cell Biology·Mehrnaz Azami-MovahedMohsen Nemat-Gorgani
Jun 20, 2013·International Journal of Biological Macromolecules·Rahim AminiSeifollah Bahramikia
Feb 28, 2014·BMC Pharmacology & Toxicology·José Antonio Vázquez
Oct 4, 2016·Frontiers in Molecular Biosciences·Ivana SirangeloClara Iannuzzi
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Nov 30, 2012·Journal of Cellular Physiology·Annalisa VilasiIvana Sirangelo
Jun 11, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Masoomeh KhalifehAmirhossein Sahebkar
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