Effect of Trichoderma harzianum on maize rhizosphere microbiome and biocontrol of Fusarium Stalk rot

Scientific Reports
Kandasamy SaravanakumarJie Chen

Abstract

Fusarium stalk rot (FSR) caused by Fusarium graminearum (FG) significantly affects the productivity of maize grain crops. Application of agrochemicals to control the disease is harmful to environment. In this regard, use of biocontrol agent (BCA) is an alternative to agrochemicals. Although Trichoderma species are known as BCA, the selection of host-pathogen specific Trichoderma is essential for the successful field application. Hence, we screened a total of 100 Trichoderma isolates against FG, selected Trichoderma harzianum (CCTCC-RW0024) for greenhouse experiments and studied its effect on changes of maize rhizosphere microbiome and biocontrol of FSR. The strain CCTCC-RW0024 displayed high antagonistic activity (96.30%), disease reduction (86.66%), biocontrol-related enzyme and gene expression. The root colonization of the strain was confirmed by eGFP tagging and qRT-PCR analysis. Pyrosequencing revealed that exogenous inoculation of the strain in maize rhizosphere increased the plant growth promoting acidobacteria (18.4%), decreased 66% of FG, and also increased the plant growth. In addition, metabolites of this strain could interact with pathogenicity related transcriptional cofactor FgSWi6, thereby contributing to its inhi...Continue Reading

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Citations

Feb 22, 2021·Current Microbiology·Olubukola O BabalolaNicholas O Igiehon
Apr 27, 2021·World Journal of Microbiology & Biotechnology·Flavia V Ferreira, Matías A Musumeci
Apr 12, 2021·Journal of Microbiological Methods·P PriyaK Harikrishnan

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Methods Mentioned

BETA
electrophoresis
column chromatography
NMR
PCR

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Discovery Studio
Studio
Discover Studio
MS Excel
ClustalW
ArgusLab
SPSS
HemI : A Toolkit for Illustrating Heatmaps
ChemSketch
MODELLER

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