Effect of Trimethyltin Chloride on Slow Vacuolar (SV) Channels in Vacuoles from Red Beet (Beta vulgaris L.) Taproots

PloS One
Zenon TrelaWaldemar Karcz

Abstract

In the present study, patch-clamp techniques have been used to investigate the effect of trimethyltin chloride (Met3SnCl) on the slow vacuolar (SV) channels in vacuoles from red beet (Beta vulgaris L.) taproots. Activity of SV channels has been measured in whole-vacuole and cytosolic side-out patch configurations. It was found that addition of trimethyltin chloride to the bath solution suppressed, in a concentration-dependent manner, SV currents in red beet vacuoles. The time constant, τ, increased significantly in the presence of the organotin. When single channel activity was analyzed, only little channel activity could be recorded at 100 μM Met3SnCl. Trimethyltin chloride added to the bath medium significantly decreased (by ca. threefold at 100 μM Met3SnCl and at 100 mV voltage, as compared to the control medium) the open probability of single channels. Single channel recordings obtained in the presence and absence of trimethyltin chloride showed that the organotin only slightly (by <10%) decreased the unitary conductance of single channels. It was also found that Met3SnCl significantly diminished the number of SV channel openings, whereas it did not change the opening times of the channels. Taking into account the above and...Continue Reading

References

Nov 6, 1992·Science·A BertlE MacRobbie
Sep 1, 1991·The Journal of Cell Biology·G P DowneyG S Worthen
Dec 1, 1994·Ecotoxicology and Environmental Safety·A Fargasová
Mar 1, 1997·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·J GabrielskaS Przestalski
Apr 8, 1998·Biochemistry and Molecular Biology International·H KleszczyńskaS Przestalski
Jan 23, 1999·The Journal of Membrane Biology·O R DobrovinskayaI I Pottosin
Feb 13, 2001·FEBS Letters·P W van den WijngaardA H de Boer
Aug 2, 2001·Bulletin of Environmental Contamination and Toxicology·Z H Song, G L Huang
Dec 26, 2001·Biochimica Et Biophysica Acta·José J ChicanoFrancisco J Aranda
Mar 13, 2003·European Biophysics Journal : EBJ·Armando Carpaneto
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Bronwyn J. Barkla, Omar Pantoja
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Christoph von BallmoosPeter Dimroth
May 9, 2006·Marine Environmental Research·Karin VeltmanA Jan Hendriks
Mar 16, 2007·Journal of Experimental Botany·I I Pottosin, G Schönknecht
Oct 28, 2008·Journal of Environmental Management·Gaëtane LespesLaurence Denaix
May 12, 2009·Cell Biology International·Romuald ZyłkaStanislaw Przestalski
Mar 2, 2010·FEBS Letters·Stanislav IsayenkovFrans J M Maathuis
Apr 1, 2010·European Biophysics Journal : EBJ·Janusz MiśkiewiczWaldemar Karcz
Mar 8, 2011·Cell Calcium·Edgar Peiter
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Nicoletta Rascio, Flavia Navari-Izzo
Apr 5, 2011·Molecular Plant·Rainer Hedrich, Irene Marten
Oct 4, 2011·Neurotoxicology and Teratology·Jiangfei ChenQiaoxiang Dong
Dec 14, 2011·Ecotoxicology and Environmental Safety·Dorota Bonarska-KujawaStanisław Przestalski
Oct 18, 2012·Physiological Reviews·Rainer Hedrich
Oct 30, 2012·Journal of Experimental Botany·Paul Vijay Kanth GutlaArmando Carpaneto
Nov 6, 2012·Biological & Pharmaceutical Bulletin·Yaichiro Kotake
Dec 13, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Alessandra PagliaraniVittoria Ventrella
Feb 25, 2014·Journal of Plant Physiology·Igor Pottosin, Oxana Dobrovinskaya
May 6, 2014·World Journal of Microbiology & Biotechnology·Przemysław BernatJerzy Długoński
Jan 1, 2013·Plants·Gerald Schönknecht

❮ Previous
Next ❯

Citations

Jul 16, 2020·International Journal of Molecular Sciences·Zbigniew BurdachWaldemar Karcz

❮ Previous
Next ❯

Software Mentioned

FitMaster
- Master
STATISTICA
Patch

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.