Effect of tRNA on the Maturation of HIV-1 Reverse Transcriptase

Journal of Molecular Biology
Tatiana V IlinaRieko Ishima

Abstract

The mature HIV-1 reverse transcriptase is a heterodimer that comprises 66 kDa (p66) and 51 kDa (p51) subunits. The latter is formed by HIV-1 protease-catalyzed removal of a C-terminal ribonuclease H domain from a p66 subunit. This proteolytic processing is a critical step in virus maturation and essential for viral infectivity. Here, we report that tRNA significantly enhances in vitro processing even at a substoichiometric tRNA:p66/p66 ratio. Other double-stranded RNAs have considerably less pronounced effect. Our data support a model where interaction of p66/p66 with tRNA introduces conformational asymmetry in the two subunits, permitting specific proteolytic processing of one p66 to provide the mature RT p66/p51 heterodimer.

Citations

Jan 17, 2020·Nucleic Acids Research·Phuong D M NguyenDonald H Burke
Dec 19, 2020·International Journal of Molecular Sciences·Tatiana V IlinaRieko Ishima

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