PMID: 3768346Sep 9, 1986Paper

Effect of trypsin treatment on the heparin- and receptor-binding properties of human plasma low-density lipoproteins

Biochemistry
A D CardinR L Jackson

Abstract

The effect of trypsin treatment on the heparin- and receptor-binding properties of human plasma low-density lipoproteins (LDL) was examined. LDL were treated with trypsin (2% by weight) for 16 h at 37 degrees C, and the trypsinized core particles (T-LDL) were isolated by gel permeation chromatography on Sepharose CL-4B. Trypsin degraded the apolipoprotein B moiety (Mr = 550,000) of LDL into numerous peptides of Mr less than 110,000, resulting in the release of 25% +/- 5% (n = 6) of its surface-associated protein. Relative to LDL, T-LDL had an increased phospholipid/protein ratio, decreased flotation density and alpha-helical structure, and increased fluidity of the surface and core constituents. Compared to LDL, T-LDL showed a 60% decreased capacity to suppress [1-14C]acetate incorporation into cellular sterols consistent with decreased binding to the LDL receptor. In contrast, T-LDL showed an enhanced capacity to form soluble complexes with heparin in the absence and presence of 2 mM Ca2+. Between 5 and 25 mM Ca2+, both LDL and T-LDL were maximally precipitated by heparin; the stoichiometry of the insoluble complexes (uronic acid/phospholipid, w/w) was 0.054 +/- 0.004 and 0.055 +/- 0.005 (n = 18) for LDL and T-LDL, respectivel...Continue Reading

References

Aug 9, 1977·Biochimica Et Biophysica Acta·J D MorrisettA M Gotto
Apr 4, 1986·Science·M S Brown, J L Goldstein
Jan 31, 1985·Biochemical and Biophysical Research Communications·M Tikkanen, G Schonfeld
Sep 20, 1983·Biochimica Et Biophysica Acta·P A DavisC B Blum
Mar 1, 1984·Analytical Biochemistry·A D CardinR L Jackson
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R W MahleyK S Holcombe
Jul 16, 1982·Biochemical and Biophysical Research Communications·S RanganathanR J Jackson
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR

❮ Previous
Next ❯

Citations

Apr 1, 1990·Chemistry and Physics of Lipids·D B Fenske, R J Cushley
Jul 29, 1988·Biochemical and Biophysical Research Communications·A D CardinJ L Krstenansky
Sep 12, 1998·Biochimica Et Biophysica Acta·S LauraeusP K Kinnunen
Nov 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·I J EdwardsJ S Parks
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A D Cardin, H J Weintraub

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Related Papers

Biulleten' eksperimental'noĭ biologii i meditsiny
I M KarmanskiĭV O Shpikiter
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
L Chan
© 2021 Meta ULC. All rights reserved