Effect of tryptase on mouse brain microvascular endothelial cells via protease-activated receptor 2

Journal of Neuroinflammation
Qin ZhouYan-Ning Qian

Abstract

Mast cells (MCs), the 'first responders' in brain injury, are able to disrupt the blood-brain barrier (BBB), but the underlying mechanism is not well understood. Tryptase is the most abundant MC secretory product. Protease-activated receptor 2 (PAR-2) has been identified as a specific receptor for tryptase, which is abundantly expressed in brain microvascular endothelial cells. The BBB comprises brain microvascular endothelial cells that display specialised molecular properties essential for BBB function and integrity. Therefore, the purpose of the present study was to investigate the effects of tryptase on mouse brain microvascular endothelial cell line bEnd3 and its potential mechanisms of action. Induction of mouse brain microvascular endothelial cell activation by tryptase was examined. Then, mouse brain microvascular endothelial cells were pretreated with a PAR-2 antagonist and stimulated with tryptase. Cellular activation, proinflammatory cytokine production, expression of PAR-2, Toll-like receptors (TLRs) and mitogen-activated protein kinases (MAPK), nuclear factor kappa B (NF-kappa B) phosphorylation were assessed. Tryptase upregulated the production of VCAM-1, MMPs (MMP9 and MMP2), TLR4 and TNF-α and downregulated the ...Continue Reading

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Citations

Aug 18, 2020·Mediators of Inflammation·Xiang ZhangJun Zhang
Mar 7, 2019·Frontiers in Cellular Neuroscience·Duraisamy KempurajAsgar Zaheer

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Methods Mentioned

BETA
electrophoresis
PCR
ELISA

Software Mentioned

NIH ImageJ
GraphPad
GraphPad Prism
Image Lab

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