Abstract
The effect of glycosylation on the assembly and antigenicity of HLA antigens was investigated by examining HLA antigens synthesized in the presence of the antibiotic tunicamycin, an inhibitor of asparagine-linked oligosaccharide addition, with monoclonal antibodies specific for a variety of antigenic determinants. The monoclonal antibody Q5/13 reactive with a determinant expressed on the beta chain of human Ia-like antigens immunoprecipitated alpha and beta subunits with reduced apparent molecular weights from tunicamycin-treated cells, indicating that glycosylation is not required for association of the Ia-like antigen alpha and beta subunits. Immunoprecipitation of HLA-A,B,C antigens from tunicamycin-treated cells with four monoclonal antibodies specific for the heavy chain and one specific for beta 2-microglobulin showed that the heavy-chain determinant detected by the antibody Q6/64 is absent from the non-glycosylated molecule. This is the first demonstration that carbohydrate addition during biosynthesis affects the protein conformation of the HLA-A,B,C heavy chain.
References
Dec 1, 1979·Cell·M S KrangelJ L Strominger
Jul 21, 1977·Nature·T A SpringerJ L Strominger
Apr 25, 1977·Biochemical and Biophysical Research Communications·N KoideT Muramatsu
May 1, 1978·Cell·C J BarnstableA Ziegler
Jul 16, 1971·Biochemical and Biophysical Research Communications·J P SegrestV T Marchesi
May 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P CresswellJ L Strominger
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Aug 15, 1970·Nature·U K Laemmli
Jan 1, 1980·The Journal of Experimental Medicine·D A Shackelford, J L Strominger
Apr 30, 1980·Virology·G KaluzaR T Schwarz
Jan 1, 1981·Immunogenetics·V QuarantaS Ferrone
Dec 1, 1980·Molecular Immunology·L E WalkerR A Reisfeld
Feb 1, 1958·Proceedings of the National Academy of Sciences of the United States of America·G F Springer, N J Ansell
Citations
Jan 1, 1981·Immunogenetics·M A PellegrinoS Ferrone
Jan 1, 1981·Immunogenetics·V QuarantaS Ferrone
Nov 1, 1984·Molecular Immunology·W L Cleveland, B F Erlanger
Dec 1, 1990·Journal of Immunogenetics·T CrepaldiP Richiardi
May 1, 1985·European Journal of Immunology·B AxelssonP Perlmann
Jul 1, 1984·Clinical Immunology and Immunopathology·M C Glassy, S Ferrone