PMID: 3521742Jun 26, 1986

Effect of tunicamycin on thiamine transport in Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
K NosakaA Iwashima


The activity of thiamine transport in Saccharomyces cerevisiae was decreased by the treatment with tunicamycin without affecting the growth of yeast cells. Although the total activity of a soluble thiamine-binding protein in yeast periplasm, which is known to be a glycoprotein, was decreased by tunicamycin treatment, the activity of thiamine uptake by yeast protoplasts was inhibited as much as by whole cells. Furthermore, tunicamycin decreased the activity of the membrane-bound thiamine-binding protein in a dose dependent way and in parallel with the thiamine transport activity. These findings suggested that the membrane-bound thiamine-binding protein is a glycoprotein which plays a functional role in thiamine transport in S. cerevisiae.


Dec 1, 1975·Biochimica Et Biophysica Acta·A IwashimaY Nose
Mar 27, 1979·Biochimica Et Biophysica Acta·A Iwashima, H Nishimura
Nov 2, 1979·Biochimica Et Biophysica Acta·A IwashimaY Nose
Dec 13, 1973·Biochimica Et Biophysica Acta·A IwashimaY Nose

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Thiamine-binding protein
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