Effect of two cleaning processes for bone allografts on gentamicin impregnation and in vitro antibiotic release

Cell and Tissue Banking
D C Coraça-HuberM Nogler

Abstract

Bone allografts are a useful and sometimes indispensable tool for the surgeon to repair bone defects. Microbial contamination is a major reason for discarding allografts from bone banks. To improve the number of safe allografts, we suggest chemical cleaning of the grafts followed by antibiotic impregnation. Comparison of two chemical cleaning processes for bone allografts aiming for antibiotic impregnation and consequently delivery rates in vitro. Bone chips of 5-10 mm were prepared from human femoral heads. Two cleaning methods (cleaning A and cleaning B) based on solutions containing hydrogen peroxide, paracetic acid, ethanol and biological detergent were carried out and compared. After the cleaning processes, the bone chips were impregnated with gentamicin. Bacillus subtilis bioassay was used to determine the gentamicin release after intervals of 1-7 days. Differences were compared with non-parametric Mann-Whitney U tests. The zones of inhibition obtained from the bone grafts cleaned with both cleaning processes were similar between the groups. The concentration of the released antibiotic was decreasing gradually over time, following a similar pattern for both groups. The cleaning procedure A as well as the cleaning procedur...Continue Reading

References

Jan 1, 1983·American Journal of Nephrology·G R MatzkeH S Shapiro
Jan 1, 1997·The Journal of Bone and Joint Surgery. British Volume·R L DeijkersM R Veen
Feb 1, 2000·Biologicals : Journal of the International Association of Biological Standardization·A PrussG Pauli
Sep 12, 2000·The Journal of Antimicrobial Chemotherapy·H WinklerA Georgopoulos
Jan 12, 2001·Clinical Orthopaedics and Related Research·J I SorgerH J Mankin
Jan 31, 2002·Acta Orthopaedica Scandinavica·H van de BeltH J Busscher
Mar 7, 2003·Journal of Orthopaedic Trauma·Shuji IsefukuA Hamish R W Simpson
Sep 17, 2003·The American Journal of Sports Medicine·Scott A Barbour, Warren King
Oct 1, 2003·The Journal of Bone and Joint Surgery. British Volume·A W BlomG C Bannister
Nov 24, 2004·Clinical Orthopaedics and Related Research·Bryan D SpringerDavid J Jacofsky
Dec 21, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C Melinda StevensJon T Mader
Jun 17, 2005·Biomaterials·Adam M ButlerWilliam Robert Walsh
Oct 1, 2005·Acta Orthopaedica·Eivind WitsøKåre Bergh
Sep 26, 2007·Biologicals : Journal of the International Association of Biological Standardization·Suvi HaimiRiitta Suuronen
Dec 7, 2007·The Journal of Bone and Joint Surgery. American Volume·Javad ParviziWilliam J Hozack
Jul 16, 2008·Acta Orthopaedica·Javad ParviziMichael A Mont
Jun 17, 2009·Archives of Orthopaedic and Trauma Surgery·N BormannBritt Wildemann
Jul 20, 2011·The Journal of Bone and Joint Surgery. British Volume·D PutzerM Nogler
Jan 4, 2012·Cell and Tissue Banking·Thomas SchubertOlivier Cornu
Jan 10, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Debora C Coraça-HubérMichael Nogler
Feb 9, 2012·Chemotherapy Research and Practice·Kristina Nadrah, Franc Strle

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Citations

May 6, 2014·The Journal of Arthroplasty·David PutzerMichael Nogler
Oct 27, 2015·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Kosaku SawadaRichard J Miron
May 29, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Putzer DavidNogler Michael
Oct 11, 2013·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Débora C Coraça-HuberMichael Nogler
May 7, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·David PutzerMichael Nogler
Mar 31, 2016·Biopreservation and Biobanking·Alexander WurmDébora C Coraça-Huber
Dec 11, 2014·Cell and Tissue Banking·Simin Dashti-KhavidakiSeyed Kazem Hosseini
Jul 8, 2017·Biopreservation and Biobanking·David PutzerMichael Nogler
Nov 9, 2019·Journal of Bone and Joint Infection·Arne PeetersLieven Thorrez
Apr 17, 2019·Materials·Elisabeth BeuttelBritt Wildemann

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