Effect of type II collagen in chondrocyte response to TGF-beta 1 regulation

Experimental Cell Research
W N Qi, S P Scully

Abstract

The in vivo role of the extracellular matrix and the manner in which it interfaces with soluble regulators remains unknown. This study reports the modulation by extracellular type II collagen of TGF-beta 1-stimulated DNA synthesis, proteoglycan synthesis, and mRNA expression for alpha 1(II) procollagen and aggrecan core protein in the adult articular chondrocyte. Bovine chondrocytes were isolated and resuspended in alginate beads which contained increasing amounts of type II collagen from 0 to 1.5% (w/v). Cultures were maintained for 7 days in basal, DMEM, TGF-beta 1 (10 ng/ml), or FBS (10%) supplemented medium. DNA and proteoglycan synthesis were determined by radiotracer incorporation. The relative amounts of mRNA were analyzed by Northern blot analysis. Exogenous collagen increased DNA synthesis in all culture conditions beginning at concentrations of 0.75% (w/v). We observed that extracellular type II collagen augments both TGF-beta 1 stimulated increases of aggrecan gene expression up to 400% and alpha 1(II) procollagen gene expression up to 180% in a dose-dependent fashion. This is distinct from cultures which were either basal or FBS supplemented medium which lacked a dose-dependent change in aggrecan gene expression and...Continue Reading

References

Dec 1, 1992·The Journal of Cell Biology·M B Sporn, A B Roberts
Jan 1, 1989·Connective Tissue Research·J F GuoD K MacCallum
Apr 15, 1983·Biochimica Et Biophysica Acta·R A KatzR V Guntaka
Jan 1, 1982·Methods in Enzymology·E J Miller, R K Rhodes
Aug 1, 1993·Experimental Cell Research·H RamdiM Lièvremont
Jan 1, 1993·The Journal of Cell Biology·C H StreuliR Derynck
Feb 1, 1993·The Journal of Bone and Joint Surgery. American Volume·S B TrippelS R Doctrow
Sep 1, 1995·Osteoarthritis and Cartilage·S W O'DriscollJ S Fitzsimmons
Mar 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J J SavareseS P Scully
Jul 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·W N Qi, S P Scully

❮ Previous
Next ❯

Citations

Mar 12, 2002·Tissue Engineering·Torsten BlunkLisa E Freed
May 24, 2007·Tissue Engineering·Sandra HofmannLorenz Meinel
Oct 2, 2008·Tissue Engineering. Part a·H Janice LeeJennifer H Elisseeff
Jun 10, 2010·American Journal of Physiology. Endocrinology and Metabolism·Tecla M TemuChanika Phornphutkul
Jul 20, 2005·Clinical and Experimental Pharmacology & Physiology·Shizuko IchinoseMotoki Tagami
Dec 21, 2005·Pathologie-biologie·L GaloisD Mainard
Jan 1, 2004·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Leon Y LumJennifer H Elisseeff
Oct 21, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Min Sung ParkJin Woo Lee
Jan 22, 2000·Arthritis and Rheumatism·S R TewC W Archer
Sep 16, 2004·Journal of Biomedical Materials Research. Part a·L Saad, M Spector
Feb 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Wen-ning Qi, Sean P Scully
Feb 21, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jin Woo LeeSean P Scully
Sep 20, 2011·Journal of Cellular Physiology·Cordula Surmann-SchmittMichael Stock
Feb 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·W N Qi, S P Scully
May 26, 2010·Journal of Materials Science. Materials in Medicine·Guillaume R RagetlyYong Sik Chung
Aug 4, 2007·The Journal of Bone and Joint Surgery. British Volume·J H LeeC W Han
Feb 3, 2005·Journal of Spinal Disorders & Techniques·Tetsuji InoueKatsumasa Takagi
Jan 18, 2005·Cell and Tissue Research·Darko BosnakovskiToru Fujinaga
Oct 18, 2001·Clinical Orthopaedics and Related Research·S P ScullyW Qi
Mar 21, 2009·Journal of Biomedical Materials Research. Part a·Yu-Hui TsaiJohn F Bowley
Dec 28, 1999·Microbes and Infection·M H Branton, J B Kopp
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·C W ChenW F Lai
Jul 11, 2006·Osteoarthritis and Cartilage·N D LeipzigK A Athanasiou
Aug 28, 2021·Experimental Eye Research·Ho Lam WongYau Kei Chan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
W N Qi, S P Scully
Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
D S MencheM I Pitman
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
W N Qi, S P Scully
© 2022 Meta ULC. All rights reserved