Effect of ultrasound on DNA polymerase reactions: monitoring on a 27-MHz quartz crystal microbalance

Biomacromolecules
Yu HoshinoYoshio Okahata

Abstract

Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k(1)) and the increase of the dissociation rate constant (k(-)(1)). The catalytic elongation rate (k(cat)) was increased, but the stability of the KF/DNA/monomer ternary complex (K(m)) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.

References

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Citations

Sep 11, 2010·Journal of the American Chemical Society·Yu HoshinoKenneth J Shea
Dec 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yu HoshinoKenneth J Shea
Mar 28, 2009·Trends in Biotechnology·Ekaterina V RokhinaJurate Virkutyte
Nov 22, 2008·Biosensors & Bioelectronics·H SunY S Fung
Aug 4, 2011·Journal of Molecular Recognition : JMR·Bernd Becker, Matthew A Cooper
Apr 16, 2015·Applied Biochemistry and Biotechnology·Daniela RemonattoDébora de Oliveira
Nov 5, 2014·Bioprocess and Biosystems Engineering·Claudia M TrentinJ Vladimir Oliveira
Aug 24, 2017·World Journal of Microbiology & Biotechnology·Shamraja S Nadar, Virendra K Rathod
Jul 14, 2019·The Science of the Total Environment·Min ZhengZhiguo Yuan
Oct 24, 2008·Journal of the American Chemical Society·Yu HoshinoKenneth J Shea
Jan 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Teruhiko MatsubaraToshinori Sato

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