PMID: 6989601Feb 1, 1980Paper

Effect of ultraviolet irradiation on 30-S ribosomal subunits. Identification of the RNA region crosslinked to protein S7

European Journal of Biochemistry
B EhresmannJ P Ebel

Abstract

The effects of ultraviolet irradiation on Escherichia coli 30-S ribosomal subunits were studied. At the doses of radiation used in this work (0-4.5 x 10(5) quanta/30-S subunit), only protein S7 was found to be significantly crosslinked to the 16-S RNA. In conditions where 25% of the protein was covalently crosslinked, the ability of the irradiated 30-S subunits to reassociate with 50-S subunits and their activity in polyphenylalanine synthesis decreased strongly. Similar results were obtained by irradiation with a germicide lamp (254 nm) or with a monochromatic ultraviolet light at 248 nm. No additional proteins were crosslinked to the 16-S RNA by irradiating 30-S subunits depleted in protein S1 or 70-S ribosomes. The covalent complex of 16-S RNA and protein S7 was isolated and digested by T1 ribonuclease. The oligonucleotide remaining attached to the crosslinked protein was characterised as A-C-C-U-C-G [position 1261 - 1266, see the sequence published by Carbon et al. (1979) Eur. J. Biochem. 160, 399-410]. Analysis of this fragment suggests that protein S7 was linked to the cytosine at position 1265 in the RNA sequence.

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Citations

Jan 20, 1981·Molecular and Cellular Biochemistry·G R KunkelH G Martinson
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
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Nov 1, 1981·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·N Giocanti, B Ekert
Apr 24, 1981·Science·H F Noller, C R Woese

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