Effect of using RNA interference to alter iNOS gene expression on the proliferation of tongue squamous cell carcinoma cell line Tca8113

The British Journal of Oral & Maxillofacial Surgery
Wei-liang ChenYong-jie Wang

Abstract

This study used RNA interference (RNAi) to explore the effect of NO and inducible nitric oxide synthase (iNOS) on apoptosis and proliferation in the tongue squamous carcinoma cell line Tca8113. Tca8113 cells were transfected with the plasmid pGenesil-1, which expresses iNOS short hairpin RNA (shRNA), or the negative control plasmid pSilencer-HK, and the transfected cells were compared with untransfected cells. The expression of iNOS was detected by histochemistry, and apoptosis was detected by flow cytometry. The expression of iNOS was significantly lower in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. The apoptosis rate was significantly higher in the pSilencer-iNOS group than in the pSilencer-HK and empty control groups. Growth monitoring showed that proliferation was also inhibited in cells transfected with pSilencer-iNOS. RNAi gene silencing decreased iNOS gene expression, induced apoptosis, and suppressed proliferation in Tca8113 cells.

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Citations

May 10, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·You-yuan WangJin-Song Li
Aug 12, 2009·The British Journal of Oral & Maxillofacial Surgery·P A BrennanL Cascarini
Aug 6, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·João Paulo Silva ServatoAdriano Mota Loyola
Jan 6, 2017·Journal of the Royal Society, Interface·Justine Dattani, Mauricio Barahona

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