Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests

Andrologia
Ali Reza TalebiJ Ghasemzadeh

Abstract

Varicocele occurs in approximately 15% to 20% of the general male population and it is the most common cause of poor semen production and decreased semen quality. It has been demonstrated that patients with varicocele have a significantly higher DNA fragmentation index (DFI) and spermatozoa with nuclear anomalies than healthy fertile men. Therefore, the aim of this study was to evaluate sperm chromatin integrity in these patients. Sixty men referring to the andrology laboratory were categorised into three different groups: 20 infertile men with varicocele, 20 infertile men with abnormal semen parameters and 20 fertile men who had normal spermatogram were considered as control group. Semen analysis was performed according to WHO criteria. To evaluate sperm chromatin quality and DNA integrity, after fixation of sperm smears, aniline blue, toluidine blue, chromomycin A(3) and acridine orange staining were applied in three groups. The slides were analysed by light and fluorescent microscopy and to determine the percentage of mature or immature spermatozoa, 200 spermatozoa were counted in each slide. The results showed that the rates of aniline blue-reacted spermatozoa were significantly higher in infertile and varicocele patients t...Continue Reading

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Citations

Jul 3, 2013·The Journal of Spinal Cord Medicine·Ali Reza TalebiNasim Tabibnejad
Dec 4, 2012·International Journal of Molecular Sciences·Clara González-MarínRosa Roy
Jan 20, 2016·Asian Journal of Andrology·Ahmad MajzoubAshok Agarwal
Jul 20, 2012·Reproductive Biomedicine Online·Ying-Jun WangWei-Li Zhang
Nov 1, 2011·Fertility and Sterility·Armand Zini, Gert Dohle
Feb 5, 2011·Fertility and Sterility·Ahmed SadekTaymour Mostafa
May 26, 2009·Gynécologie, obstétrique & fertilité·P Marchetti, C Marchetti
Sep 16, 2014·Andrologia·W-J Zhu, J Li
Aug 16, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Ali Reza TalebiIman Halvaei
Jul 23, 2013·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Marzieh RahimipourMarjan Omidi
May 13, 2018·BJU International·Parisa MohammadiMohammad H Nasr-Esfahani
Aug 11, 2017·Clinical and Experimental Reproductive Medicine·Fatemeh DehghanpourAli Reza Talebi
Oct 4, 2019·Systems Biology in Reproductive Medicine·Miriama ŠtiavnickáJan Nevoral
Mar 28, 2017·Systems Biology in Reproductive Medicine·Marziyeh TavalaeeMohammad H Nasr-Esfahani
Oct 5, 2018·Journal of Endocrinological Investigation·M ZavattaroF Lanfranco
Aug 7, 2018·The World Journal of Men's Health·Yong Seog ParkJu Tae Seo
Apr 18, 2018·Clinical and Experimental Reproductive Medicine·Tahereh RahiminiaAli Reza Talebi
Jul 24, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Monir TaherimoghaddamFaeze Foroughi-Parvar

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