PMID: 8941923Dec 1, 1996Paper

Effect of vasopressin on intracellular Na+ concentration in cortical collecting duct

Kidney International. Supplement
M D Breyer, D Fredin

Abstract

Vasopressin stimulates transepithelial Na+ absorption in the rabbit cortical collection duct (CCD), however, this increase is short-lived and is followed by sustained inhibition of Na+ absorption. Previous studies suggest that increased basolateral Ca2+ influx via a Na+/Ca2+ exchanger may be involved in feedback inhibition of Na+ absorption, accounting for this transient stimulation. The present studies used the Na+ sensitive dye, SBFI, to measure intracellular NA+ ([Na+]i) in the CCD to determine whether either vasopressin or 3'5'cAMP increase cell Na+ in the CCD, thereby increasing Na+/Ca2+ exchange. Resting CCD [Na+]i was 23 +/- 2.5 mM. Both arginine vasopressin (AVP, 23 pM) and 0.1 mM 8-chlorophenylthio-cAMP (8CPTcAMP) transiently increased [Na+]i, which peaked within approximately 250 seconds of their addition and then gradually fell towards baseline. AVP increased [Na+]i from 19.4 +/- 3.6 to a peak of 44.4 +/- 14 mM and 8CPTcAMP increased [Na+]i from 11.8 +/- 3.6 to a peak of 38.7 +/- 6.2 mM. This [Na+]i increase was completely blocked by luminal Na+ removal. Inhibition of the basolateral Na/K ATPase with 10 microM ouabain caused [Na+]i to increase to 71.8 +/- 11.6 mM. These results demonstrate that cAMP and AVP increase ...Continue Reading

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