PMID: 8954137Nov 22, 1996Paper

Effect of vitamin E on expression of cyclooxygenase-2 in lipopolysaccharide-stimulated rat macrophages

Biochimica Et Biophysica Acta
W SakamotoS Yamamoto

Abstract

To clarify the role of vitamin E (alpha-tocopherol) for the induction of cyclooxygenase-2 (COX-2) in rat macrophages stimulated by lipopolysaccharide (LPS), vitamin E-enriched macrophages were prepared by intraperitoneal injection of vitamin E for 6 days at a rate of 5 mg per day. The production of PGE2 was increased in dose- and time-dependent manners by addition of LPS in both control and vitamin E-enriched peritoneal macrophages. The maximum effect of LPS was observed in 12 h at concentration of 5 micrograms/ml. By analyzing COX-2 mRNA level by Northern blot and COX-2 enzyme mass and phosphotyrosine by Western blot, it was revealed that the increase of PGE2 production reflected the induction of COX-2 expression through activation of tyrosine kinase. Vitamin E failed to inhibit PGE2 production in LPS-stimulated macrophages; however, genistein, a tyrosine kinase inhibitor, completely inhibited the production at 100 microM. These results suggest that vitamin E does not inhibit COX-2 expression via LPS-mediated tyrosine kinase signal transduction pathway.

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T Hla, K Neilson
Jul 8, 1991·Biochimica Et Biophysica Acta·W SakamotoM Mino
Sep 1, 1990·The American Journal of Clinical Nutrition·S N MeydaniJ B Blumberg
Jul 1, 1987·Chemistry and Physics of Lipids·E Niki
Nov 3, 1993·Biochimica Et Biophysica Acta·W SakamotoH Handa
May 7, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Boyer D Winters, James M Reid

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Citations

Dec 30, 1999·Immunopharmacology·W SakamotoJ Nishihira
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·Q JiangB N Ames
Mar 2, 2011·Pharmacology & Therapeutics·Nam Joo KangKi Won Lee
Jul 11, 2006·BioFactors·Ki Won Lee, Hyong Joo Lee
Mar 8, 2006·Mechanisms of Ageing and Development·Ki Won Lee, Hyong Joo Lee
Nov 18, 2000·The Journal of Laboratory and Clinical Medicine·T OriguchiK Eguchi
Jun 9, 2001·Experimental Biology and Medicine·B A WatkinsM F Seifert
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sergejs BerdnikovsJoan M Cook-Mills
Sep 18, 1998·Biochimica Et Biophysica Acta·W SakamotoS Yukawa

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