Effect of Vitamins D and E on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An In Vitro Study

International Journal of Dentistry
Lina M EscobarMaría-Clara González

Abstract

The aim of the present study was to determine the effects of vitamins D and E on the proliferation, morphology, and differentiation of human dental pulp stem cells (hDPSCs). In this in vitro experimental study, hDPSCs were isolated, characterized, and treated with vitamins D and E, individually and in combination, utilizing different doses and treatment periods. Changes in morphology and cell proliferation were evaluated using light microscopy and the resazurin assay, respectively. Osteoblast differentiation was evaluated with alizarin red S staining and expression of RUNX2, Osterix, and Osteocalcin genes using real-time RT-PCR. Compared with untreated cells, the number of cells significantly reduced following treatment with vitamin D (49%), vitamin E (35%), and vitamins D + E (61%) after 144 h. Compared with cell cultures treated with individual vitamins, cells treated with vitamins D + E demonstrated decreased cell confluence, with more extensive and flatter cytoplasm that initiated the formation of a significantly large number of calcified nodules after 7 days of treatment. After 14 days, treatment with vitamins D, E, and D + E increased the transcription of RUNX2, Osterix, and Osteocalcin genes. Vitamins D and E induced ost...Continue Reading

References

Oct 1, 1988·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·M K Collins, P M Sinclair
Mar 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takeshi ShibataKyoji Ikeda
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Oct 19, 2004·Journal of Bone and Mineral Metabolism·Masayoshi Kawakami, Teruko Takano-Yamamoto
Jul 14, 2006·The Journal of Oral Implantology·I J De KokL F Cooper
Sep 15, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jan H SchefeHeiko Funke-Kaiser
Mar 11, 2010·The Journal of Veterinary Medical Science·Satoshi SoetaHajime Aamsaki
Aug 21, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Rashi Khanna-JainSusanna Miettinen
Oct 2, 2012·Head & Face Medicine·Kent UrbanUNKNOWN Biomineralisation Research Unit
Oct 22, 2014·World Journal of Methodology·Angela Domínguez Camacho, Sergio Andres Velásquez Cujar
Feb 19, 2015·Journal of Bone and Mineral Metabolism·Michela BosettiMario Cannas
Dec 14, 2016·Stem Cells International·Francesca PosaGiorgio Mori
Mar 15, 2018·Journal of Periodontal & Implant Science·Hyun-Soo KimByung-Ock Kim
Apr 20, 2018·International Journal of Cell Biology·Paula A BaldiónJaime E Castellanos
Jun 26, 2018·Cytotechnology·Ibrahim Mortada, Rola Mortada
Jun 27, 2018·Drug Design, Development and Therapy·Wan Nuraini Wan HasanSoelaiman Ima-Nirwana

❮ Previous
Next ❯

Citations

Feb 5, 2021·Journal of Oral Biology and Craniofacial Research·Lina M EscobarJaime E Castellanos

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SPSS
LinRegPCR

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.