Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution

Ecology and Evolution
Toshiaki JoToshifumi Minamoto

Abstract

Environmental DNA (eDNA) analysis has successfully detected organisms in various aquatic environments. However, there is little basic information on eDNA, including the eDNA shedding and degradation processes. This study focused on water temperature and fish biomass and showed that eDNA shedding, degradation, and size distribution varied depending on water temperature and fish biomass. The tank experiments consisted of four temperature levels and three fish biomass levels. The total eDNA and size-fractioned eDNA from Japanese Jack Mackerels (Trachurus japonicus) were quantified before and after removing the fish. The results showed that the eDNA shedding rate increased at higher water temperature and larger fish biomass, and the eDNA decay rate also increased at higher temperature and fish biomass. In addition, the small-sized eDNA fractions were proportionally larger at higher temperatures, and these proportions varied among fish biomass. After removing the fish from the tanks, the percentage of eDNA temporally decreased when the eDNA size fraction was >10 µm, while the smaller size fractions increased. These results have the potential to make the use of eDNA analysis more widespread in the future.

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Citations

Jul 13, 2019·Journal of Fish Biology·Tomoya HoriuchiToshifumi Minamoto
Nov 21, 2019·Proceedings. Biological Sciences·Jori B HarrisonSean M Rogers
Oct 22, 2019·Molecular Ecology Resources·Girish KumarMichelle R Gaither
Feb 4, 2021·Scientific Reports·Nathaniel T MarshallSubba Rao Chaganti
Mar 25, 2021·Trends in Ecology & Evolution·Matthew C YatesMelania E Cristescu
May 28, 2021·Scientific Reports·Tetsu YasashimotoToshifumi Minamoto
Jul 16, 2021·The Science of the Total Environment·Mohan AmarasiriKazunari Sei
Jul 26, 2021·The Science of the Total Environment·Beilun ZhaoKrijn Trimbos
Jul 23, 2019·Environmental Science & Technology·Toshiaki JoToshifumi Minamoto
Sep 25, 2021·Scientific Reports·Kristie J SchmidtHugh B Britten

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Methods Mentioned

BETA
PCR

Software Mentioned

R Core
R

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