PMID: 6169733Sep 1, 1981Paper

Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages

The Journal of Cell Biology
B Poole, S Ohkuma

Abstract

The spectral characteristics of dextran, labeled with fluorescein, depend upon pH. We have loaded the lysosomes of mouse peritoneal macrophages with this fluorescence probe and used it to measure the intralysosomal pH under various conditions. The pH of the medium has no effect on the intralysosomal pH. Weakly basic substances in the medium cause a concentration-dependent increase in the intralysosomal pH. However, the concentration of base necessary to produce a significant change in the intralysosomal pH varies over a wide range for different bases. The active form of the base is the neutral, unprotonated form. Although most of these weak bases cause an increase in the volume of the lysosomes, increase in lysosomal volume itself causes only a minor perturbation of the intralysosomal pH. This was demonstrated in cells whose lysosomes were loaded with sucrose, and in cells vacuolated as a demonstrated in cells whose lysosomes were loaded with sucrose, and in cells vacuolated as a consequence of exposure to concanavalin A. The results of these studies are interpreted in terms of energy-dependent lysosomal acidification and leakage of protons out of the lysosomes in the form of protonated weak bases.

References

Nov 14, 1977·Biochimica Et Biophysica Acta·D J Reijngoud, J M Tager
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S Ohkuma, B Poole
Mar 30, 1979·Biochemical and Biophysical Research Communications·D L Schneider
Jun 6, 1977·The Journal of Membrane Biology·D L Schneider
Sep 15, 1974·Biochemical Pharmacology·C de DuveF Van Hoof
Jan 1, 1965·The Journal of Experimental Medicine·Z A COHN, B BENSON

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Citations

Apr 15, 1990·Biotechnology and Bioengineering·A McQueen, J E Bailey
Feb 20, 1992·Biotechnology and Bioengineering·S S OzturkB O Palsson
May 4, 2001·Molecular Reproduction and Development·C R MoralesS Lefrancois
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Mar 21, 2012·Cell Biology and Toxicology·Toshihiko AkiKoichi Uemura
Apr 28, 2012·Pharmaceutical Research·Ljubica Harhaji-TrajkovicVladimir Trajkovic
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Feb 17, 1999·Biochimica Et Biophysica Acta·V IvanenkovA G Menon
Mar 4, 2000·Clinics in Dermatology·R WolfV Ruocco

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